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Antimicrobial Agents and Chemotherapy, June 2005, p. 2246-2259, Vol. 49, No. 6
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.6.2246-2259.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transcriptional Response of Enterococcus faecalis V583 to Erythromycin

Laboratory of Microbial Gene Technology,¹ Section for Bioinformatics and Data Analysis, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway,² Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455,³ Department of Veterinary Pathobiology, Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota 55108,⁴ Center for the Study of Emerging and Re-Emerging Pathogens, The University of Texas Medical School, Houston, Texas 77030⁵

Received 1 November 2004/ Returned for modification 16 January 2005/ Accepted 27 February 2005

A transcriptional profile of Enterococcus faecalis V583 (V583) treated with erythromycin is presented. This is the first study describing a complete transcriptional profile of Enterococcus. E. faecalis is a common and nonvirulent bacterium in many natural environments, but also an important cause of nosocomial infections. We have used a genome-wide microarray based on the genome sequence of V583 to study gene expression in cells exposed to erythromycin. V583 is resistant to relatively high concentrations of erythromycin, but growth is retarded by the treatment. The effect of erythromycin treatment on V583 was studied by a time course experiment; samples were extracted at five time points over a period of 90 min. A drastic change in gene transcription was seen with the erythromycin-treated cells compared to the untreated cells. Altogether, 260 genes were down-regulated at one or more time points, while 340 genes were up-regulated. Genes encoding hypothetical proteins and genes encoding transport and binding proteins were the two most dominating groups of differentially expressed genes. The gene encoding ermB (EFA0007) was expressed, but not differentially, which indicated that other genes are important for the survival and growth maintenance of V583 treated with erythromycin. One of these genes is a putative MsrC-like protein, which was up-regulated at all time points studied. Other specific genes that were found to be up-regulated were genes encoding ABC transporters and two-component regulatory systems, and these may be genes that are important for the specific response of V583 to erythromycin.

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