This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morozumi, M.
Right arrow Articles by Ubukata, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morozumi, M.
Right arrow Articles by Ubukata, K.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2005, p. 2302-2306, Vol. 49, No. 6
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.6.2302-2306.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Emergence of Macrolide-Resistant Mycoplasma pneumoniae with a 23S rRNA Gene Mutation

Miyuki Morozumi,1 Keiko Hasegawa,1 Reiko Kobayashi,1 Nagako Inoue,2 Satoshi Iwata,3 Haruo Kuroki,4 Naohisa Kawamura,5 Eiichi Nakayama,6 Takeshi Tajima,6 Kouichi Shimizu,7 and Kimiko Ubukata1*

Laboratory of Infectious Agents Surveillance, Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minatoku, Tokyo, Japan,1 Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan,2 National Hospital Organization Tokyo Medical Center, Tokyo, Japan,3 Medical Corporation Nagatsu-kai Saito Hospital, Chiba, Japan,4 Osaka Rosai Hospital, Osaka, Japan,5 Hakujikai Memorial Hospital, Tokyo, Japan,6 Saiseikai Ibaraki Hospital, Osaka, Japan7

Received 7 October 2004/ Returned for modification 5 December 2004/ Accepted 31 January 2005

A total of 195 Mycoplasma pneumoniae strains were isolated from 2,462 clinical specimens collected between April 2002 and March 2004 from pediatric outpatients with respiratory tract infections. Susceptibilities to six macrolide antibiotics (ML), telithromycin, minocycline, levofloxacin, and sitafloxacin were determined by the microdilution method using PPLO broth. A total of 183 M. pneumoniae isolates were susceptible to all agents and had excellent MIC90s in the following order: 0.00195 µg/ml for azithromycin and telithromycin, 0.0078 µg/ml for clarithromycin, 0.0156 µg/ml for erythromycin, 0.0625 µg/ml for sitafloxacin, 0.5 µg/ml for minocycline, and 1 µg/ml for levofloxacin. Notably, 12 ML-resistant M. pneumoniae strains were isolated from patients with pneumonia (10 strains) or acute bronchitis (2 strains). These strains showed resistance to ML with MICs of ≥1 µg/ml, except to rokitamycin. Transition mutations of A2063G or A2064G, which correspond to A2058 and A2059 in Escherichia coli, in domain V on the 23S rRNA gene in 11 ML-resistant strains were identified. By pulsed-field gel electrophoresis typing, these strains were classified into groups I and Vb, as described previously (A. Cousin-Allery, A. Charron, B. D. Barbeyrac, G. Fremy, J. S. Jensen, H. Renaudin, and C. Bebear, Epidemiol. Infect. 124:103-111, 2000). These findings suggest that excessive usage of MLs acts as a trigger to select mutations on the corresponding 23S rRNA gene with the resultant occurrence of ML-resistant M. pneumoniae. Monitoring ML susceptibilities for M. pneumoniae is necessary in the future.

* Corresponding author. Mailing address: Laboratory of Infectious Agents Surveillance, Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minatoku, Tokyo, 108-8641 Japan. Phone: 81-3-5791-6385. Fax: 81-3-5791-6386. E-mail: ubukatak{at}lisci.kitasato-u.ac.jp.

Antimicrobial Agents and Chemotherapy, June 2005, p. 2302-2306, Vol. 49, No. 6
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.6.2302-2306.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hamasuna, R., Jensen, J. S., Osada, Y. (2009). Antimicrobial Susceptibilities of Mycoplasma genitalium Strains Examined by Broth Dilution and Quantitative PCR. Antimicrob. Agents Chemother. 53: 4938-4939 [Abstract] [Full Text]  
  • Peuchant, O., Menard, A., Renaudin, H., Morozumi, M., Ubukata, K., Bebear, C. M., Pereyre, S. (2009). Increased macrolide resistance of Mycoplasma pneumoniae in France directly detected in clinical specimens by real-time PCR and melting curve analysis. J Antimicrob Chemother 64: 52-58 [Abstract] [Full Text]  
  • Waites, K. B., Crabb, D. M., Duffy, L. B. (2009). Comparative In Vitro Susceptibilities of Human Mycoplasmas and Ureaplasmas to a New Investigational Ketolide, CEM-101. Antimicrob. Agents Chemother. 53: 2139-2141 [Abstract] [Full Text]  
  • Liu, Y., Ye, X., Zhang, H., Xu, X., Li, W., Zhu, D., Wang, M. (2009). Antimicrobial Susceptibility of Mycoplasma pneumoniae Isolates and Molecular Analysis of Macrolide-Resistant Strains from Shanghai, China. Antimicrob. Agents Chemother. 53: 2160-2162 [Abstract] [Full Text]  
  • Xin, D., Mi, Z., Han, X., Qin, L., Li, J., Wei, T., Chen, X., Ma, S., Hou, A., Li, G., Shi, D. (2009). Molecular Mechanisms of Macrolide Resistance in Clinical Isolates of Mycoplasma pneumoniae from China. Antimicrob. Agents Chemother. 53: 2158-2159 [Abstract] [Full Text]  
  • Wolff, B. J., Thacker, W. L., Schwartz, S. B., Winchell, J. M. (2008). Detection of Macrolide Resistance in Mycoplasma pneumoniae by Real-Time PCR and High-Resolution Melt Analysis. Antimicrob. Agents Chemother. 52: 3542-3549 [Abstract] [Full Text]  
  • Morozumi, M., Iwata, S., Hasegawa, K., Chiba, N., Takayanagi, R., Matsubara, K., Nakayama, E., Sunakawa, K., Ubukata, K., the Acute Respiratory Diseases Study Group, (2008). Increased Macrolide Resistance of Mycoplasma pneumoniae in Pediatric Patients with Community-Acquired Pneumonia. Antimicrob. Agents Chemother. 52: 348-350 [Abstract] [Full Text]  
  • Shimizu, T., Kida, Y., Kuwano, K. (2008). Mycoplasma pneumoniae-Derived Lipopeptides Induce Acute Inflammatory Responses in the Lungs of Mice. Infect. Immun. 76: 270-277 [Abstract] [Full Text]  
  • Pereyre, S., Charron, A., Renaudin, H., Bebear, C., Bebear, C. M. (2007). First Report of Macrolide-Resistant Strains and Description of a Novel Nucleotide Sequence Variation in the P1 Adhesin Gene in Mycoplasma pneumoniae Clinical Strains Isolated in France over 12 Years. J. Clin. Microbiol. 45: 3534-3539 [Abstract] [Full Text]  
  • Morozumi, M., Nakayama, E., Iwata, S., Aoki, Y., Hasegawa, K., Kobayashi, R., Chiba, N., Tajima, T., Ubukata, K., the Acute Respiratory Diseases Study Group, (2006). Simultaneous Detection of Pathogens in Clinical Samples from Patients with Community-Acquired Pneumonia by Real-Time PCR with Pathogen-Specific Molecular Beacon Probes. J. Clin. Microbiol. 44: 1440-1446 [Abstract] [Full Text]  
  • Suzuki, S., Yamazaki, T., Narita, M., Okazaki, N., Suzuki, I., Andoh, T., Matsuoka, M., Kenri, T., Arakawa, Y., Sasaki, T. (2006). Clinical Evaluation of Macrolide-Resistant Mycoplasma pneumoniae. Antimicrob. Agents Chemother. 50: 709-712 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»