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Antimicrobial Agents and Chemotherapy, June 2005, p. 2536-2538, Vol. 49, No. 6
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.6.2536-2538.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Targeted Gene Disruption of the 14- Sterol Demethylase (cyp51A) in Aspergillus fumigatus and Its Role in Azole Drug Susceptibility

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

Received 1 September 2004/ Returned for modification 8 October 2004/ Accepted 31 January 2005

The role of Aspergillus fumigatus 14-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.

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