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Antimicrobial Agents and Chemotherapy, July 2005, p. 2583-2588, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2583-2588.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Different Anti-Candida Activities of Two Human Lactoferrin-Derived Peptides, Lfpep and Kaliocin-1

Mónica Viejo-Díaz,1,2,{dagger} María T. Andrés,2,{dagger} and José F. Fierro1,2*

Department of Functional Biology (Microbiology), Faculty of Medicine,1 Laboratory of Oral Microbiology, School of Stomatology, University of Oviedo, 33006 Oviedo, Spain2

Received 15 December 2004/ Returned for modification 18 January 2005/ Accepted 4 April 2005

The synthetic peptides Lfpep and kaliocin-1 include the sequences from positions 18 to 40 and 153 to 183 of human lactoferrin, respectively. Lfpep is a cationic peptide with bactericidal and giardicidal effects, whereas kaliocin-1 is a novel bactericidal peptide that corresponds to a highly homologous sequence present in the transferrin family of proteins. Both peptides presented fungicidal activity against Candida spp., including fluconazole- and amphotericin B-resistant clinical isolates. Lfpep exhibited higher antifungal activity (8- to 30-fold) and salt resistance than kaliocin-1. The killing activity of Lfpep was mediated by its permeabilizing activity on Candida albicans cells, whereas kaliocin-1 was unable to disrupt the cytoplasmic membrane, as indicated by its inability to allow permeation of propidium iodide and the small amount of K+ released. The amino acid sequence of kaliocin-1 includes the "multidimensional antimicrobial signature" conserved in disulfide-containing antimicrobial peptides and a striking similarity to brevinin-1Sa, an antimicrobial peptide from frog skin secretions, exhibiting a "Rana box"-like sequence. These features may be of interest in the design of new antifungals.

* Corresponding author. Mailing address: Department of Functional Biology (Microbiology), Faculty of Medicine, University of Oviedo, C/ Julian Claveria, 6, 33006 Oviedo, Spain. Phone: 34-985-103643. Fax: 34-985-103533. E-mail: jffierro{at}uniovi.es.

{dagger} M.V.-D. and M.T.A. contributed equally to this report.

Antimicrobial Agents and Chemotherapy, July 2005, p. 2583-2588, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2583-2588.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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