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Antimicrobial Agents and Chemotherapy, July 2005, p. 2778-2784, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2778-2784.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Structure-Based Phylogeny of the Metallo-ß-Lactamases

Gianpiero Garau,1 Anne Marie Di Guilmi,2 and Barry G. Hall3*

Laboratoire de Cristallographie Macromoléulaire, Institut de Biologie Structural "Jean-Pierre Ebel," CEA-CNRS-UJF, F-38027 Grenoble, France,1 Laboratoire d'Ingenierie des Macromolecules, Institut de Biologie Structural "Jean-Pierre Ebel," CEA-CNRS-UJF, F-38027 Grenoble, France,2 Biology Department, University of Rochester, Rochester, New York3

Received 16 November 2004/ Returned for modification 31 January 2005/ Accepted 25 February 2005

The metallo-ß-lactamases fall into two groups: Ambler class B subgroups B1 and B2 and Ambler class B subgroup B3. The two groups are so distantly related that there is no detectable sequence homology between members of the two different groups, but homology is clearly detectable at the protein structure level. The multiple structure alignment program MAPS has been used to align the structures of eight metallo-ß-lactamases and five structurally homologous proteins from the metallo-ß-lactamase superfamily, and that alignment has been used to construct a phylogenetic tree of the metallo-ß-lactamases. The presence of genes from Eubacteria, Archaebacteria, and Eukaryota on that tree is consistent with a very ancient origin of the metallo-ß-lactamase family.

* Corresponding author. Present address: 218 Chuckanut Point Rd., Bellingham, WA 98229. E-mail: drbh{at}mail.rochester.edu.

Antimicrobial Agents and Chemotherapy, July 2005, p. 2778-2784, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2778-2784.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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