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Antimicrobial Agents and Chemotherapy, July 2005, p. 2793-2801, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2793-2801.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C

François-Xavier Weill,1* Laëtitia Fabre,1 Bernadette Grandry,2 Patrick A. D. Grimont,1 and Isabelle Casin2

Centre National de Référence des Salmonella, Unité de Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, INSERM U389, 28 rue du Docteur Roux, 75724 Paris cedex 15, France,1 Service de Microbiologie, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75010 Paris, France2

Received 24 November 2004/ Returned for modification 14 February 2005/ Accepted 25 February 2005

This study was conducted to investigate the occurrence of multiple-antibiotic resistance among 261 clinical isolates of Salmonella enterica serotype Paratyphi B strains collected between 2000 and 2003 through the network of the French National Reference Center for Salmonella. The 47 multidrug-resistant (MDR) isolates identified (18%), were characterized on the basis of the presence of several resistance genes (blaTEM, blaPSE-1, blaCTX-M, floR, aadA2, qacE{Delta}1, and sul1), the presence of Salmonella genomic island 1 (SGI1) by PCR mapping and hybridization, and the clonality of these isolates by several molecular (ribotyping, IS200 profiling, and pulsed-field gel electrophoresis [PFGE]) and phage typing methods. The results of PCR and Southern blot experiments indicated that 39 (83%) of the 47 S. enterica serotype Paratyphi B biotype Java MDR isolates possessed the SGI1 cluster (MDR/SGI1). Among these 39 MDR/SGI1 isolates, only 3 contained variations in SGI1, SGI1-B (n = 1) and SGI1-C (n = 2). The 39 MDR/SGI1 isolates showed the same specific PstI-IS200 profile 1, which contained seven copies from 2.6 to 18 kb. Two PstI ribotypes were found in MDR/SGI1 isolates, RP1 (n = 38) and RP6 (n = 1). Ribotype RP1 was also found in two susceptible strains. Analysis by PFGE using XbaI revealed that all the MDR/SGI1 isolates were grouped in two related clusters, with a similarity percentage of 82%. Isolation of MDR/SGI1 isolates in France was observed mainly between the second quarter of 2001 and the end of 2002. The source of the contamination has not been identified to date. A single isolate possessing the extended-spectrum ß-lactamase blaCTX-M-15 gene was also identified during the study.

* Corresponding author. Mailing address: Centre National de Référence des Salmonella, Unité de Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France. Phone: 33-(0)1 45 68 83 45. Fax: 33-(0)1 5 68 88 37. E-mail: fxweill{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, July 2005, p. 2793-2801, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2793-2801.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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