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Antimicrobial Agents and Chemotherapy, July 2005, p. 2979-2982, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2979-2982.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutagenesis Analysis of a Conserved Region Involved in Acetyl Coenzyme A Binding in the Aminoglycoside 6'-N-Acetyltransferase Type Ib Encoded by Plasmid pJHCMW1

Atousa Pourreza, Mavee Witherspoon, Jessica Fox, Jason Newmark, Duyen Bui, and Marcelo E. Tolmasky*

Department of Biological Science, School of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California 92834-6850

Received 30 October 2004/ Returned for modification 20 January 2005/ Accepted 15 April 2005

Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.

* Corresponding author. Mailing address: Department of Biological Science, School of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92834-6850. Phone: (714) 278-5263. Fax: (714) 278-3426. E-mail: mtolmasky{at}fullerton.edu.

Antimicrobial Agents and Chemotherapy, July 2005, p. 2979-2982, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.2979-2982.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Soler Bistue, A. J. C., Martin, F. A., Petroni, A., Faccone, D., Galas, M., Tolmasky, M. E., Zorreguieta, A. (2006). Vibrio cholerae InV117, a Class 1 Integron Harboring aac(6')-Ib and blaCTX-M-2, Is Linked to Transposition Genes.. Antimicrob. Agents Chemother. 50: 1903-1907 [Abstract] [Full Text]  


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