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Antimicrobial Agents and Chemotherapy, July 2005, p. 3004-3008, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3004-3008.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Production of Enterocin P, an Antilisterial Pediocin-Like Bacteriocin from Enterococcus faecium P13, in Pichia pastoris

Jorge Gutiérrez, Raquel Criado, María Martín, Carmen Herranz, Luis M. Cintas, and Pablo E. Hernández^*

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain

Received 24 November 2004/ Returned for modification 14 January 2004/ Accepted 25 February 2005

The gene encoding mature enterocin P (EntP), an antimicrobial peptide from Enterococcus faecium P13, was cloned into the pPICZA expression vector to generate plasmid pJC31. This plasmid was integrated into the genome of P. pastoris X-33, and EntP was heterologously secreted from the recombinant P. pastoris X-33t₁ derivative at a higher production and antagonistic activity than from E. faecium P13.

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* Corresponding author. Mailing address: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. Phone: 34-913943752. Fax: 34-913943743. E-mail: ehernan{at}vet.ucm.es.

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Antimicrobial Agents and Chemotherapy, July 2005, p. 3004-3008, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3004-3008.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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