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Antimicrobial Agents and Chemotherapy, July 2005, p. 3050-3052, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3050-3052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction of the Plasmid-Encoded Quinolone Resistance Protein QnrA with Escherichia coli Topoisomerase IV

Division of Infectious Diseases, Massachusetts General Hospital, Boston,¹ Infectious Disease Department, Lahey Clinic, Burlington, Massachusetts²

Received 3 February 2005/ Returned for modification 10 March 2005/ Accepted 14 April 2005

Purified QnrA blocked ciprofloxacin inhibition of topoisomerase IV, just as QnrA was previously found to prevent quinolone inhibition of DNA gyrase. With a gel displacement assay, tagged QnrA was shown to bind to topoisomerase IV and its subunits in a reaction that did not depend on the presence of DNA, quinolone, or ATP.

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