This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tran, J. H.
Right arrow Articles by Hooper, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tran, J. H.
Right arrow Articles by Hooper, D. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2005, p. 3050-3052, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3050-3052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction of the Plasmid-Encoded Quinolone Resistance Protein QnrA with Escherichia coli Topoisomerase IV

John H. Tran,1 George A. Jacoby,2* and David C. Hooper1

Division of Infectious Diseases, Massachusetts General Hospital, Boston,1 Infectious Disease Department, Lahey Clinic, Burlington, Massachusetts2

Received 3 February 2005/ Returned for modification 10 March 2005/ Accepted 14 April 2005

Purified QnrA blocked ciprofloxacin inhibition of topoisomerase IV, just as QnrA was previously found to prevent quinolone inhibition of DNA gyrase. With a gel displacement assay, tagged QnrA was shown to bind to topoisomerase IV and its subunits in a reaction that did not depend on the presence of DNA, quinolone, or ATP.


* Corresponding author. Mailing address: Lahey Clinic, 41 Mall Road, Burlington, MA 01805. Phone: (781) 744-2928. Fax: (781) 744-5486. E-mail: george.a.jacoby{at}lahey.org.


Antimicrobial Agents and Chemotherapy, July 2005, p. 3050-3052, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3050-3052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Minh Vien, L. T., Baker, S., Phuong Thao, L. T., Phuong Tu, L. T., Thu Thuy, C., Thu Nga, T. T., Minh Hoang, N. V., Campbell, J. I., Minh Yen, L., Trong Hieu, N., Vinh Chau, N. V., Farrar, J., Schultsz, C. (2009). High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam. J Med Microbiol 58: 1585-1592 [Abstract] [Full Text]  
  • Strahilevitz, J., Jacoby, G. A., Hooper, D. C., Robicsek, A. (2009). Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat. Clin. Microbiol. Rev. 22: 664-689 [Abstract] [Full Text]  
  • Govender, N., Smith, A. M., Karstaedt, A. S., Keddy, K. H., for the Group for Enteric, Respiratory and Meninge, (2009). Plasmid-mediated quinolone resistance in Salmonella from South Africa. J Med Microbiol 58: 1393-1394 [Full Text]  
  • Cavaco, L. M., Aarestrup, F. M. (2009). Evaluation of Quinolones for Use in Detection of Determinants of Acquired Quinolone Resistance, Including the New Transmissible Resistance Mechanisms qnrA, qnrB, qnrS, and aac(6')Ib-cr, in Escherichia coli and Salmonella enterica and Determinations of Wild-Type Distributions. J. Clin. Microbiol. 47: 2751-2758 [Abstract] [Full Text]  
  • Pallecchi, L., Riccobono, E., Mantella, A., Bartalesi, F., Sennati, S., Gamboa, H., Gotuzzo, E., Bartoloni, A., Rossolini, G. M. (2009). High Prevalence of qnr Genes in Commensal Enterobacteria from Healthy Children in Peru and Bolivia. Antimicrob. Agents Chemother. 53: 2632-2635 [Abstract] [Full Text]  
  • Rodriguez-Martinez, J. M., Briales, A., Velasco, C., Conejo, M. C., Martinez-Martinez, L., Pascual, A. (2009). Mutational analysis of quinolone resistance in the plasmid-encoded pentapeptide repeat proteins QnrA, QnrB and QnrS. J Antimicrob Chemother 63: 1128-1134 [Abstract] [Full Text]  
  • Wang, M., Guo, Q., Xu, X., Wang, X., Ye, X., Wu, S., Hooper, D. C., Wang, M. (2009). New Plasmid-Mediated Quinolone Resistance Gene, qnrC, Found in a Clinical Isolate of Proteus mirabilis. Antimicrob. Agents Chemother. 53: 1892-1897 [Abstract] [Full Text]  
  • Merens, A., Matrat, S., Aubry, A., Lascols, C., Jarlier, V., Soussy, C.-J., Cavallo, J.-D., Cambau, E. (2009). The Pentapeptide Repeat Proteins MfpAMt and QnrB4 Exhibit Opposite Effects on DNA Gyrase Catalytic Reactions and on the Ternary Gyrase-DNA-Quinolone Complex. J. Bacteriol. 191: 1587-1594 [Abstract] [Full Text]  
  • Wang, M., Jacoby, G. A., Mills, D. M., Hooper, D. C. (2009). SOS Regulation of qnrB Expression. Antimicrob. Agents Chemother. 53: 821-823 [Abstract] [Full Text]  
  • Yang, H., Chen, H., Yang, Q., Chen, M., Wang, H. (2008). High Prevalence of Plasmid-Mediated Quinolone Resistance Genes qnr and aac(6')-Ib-cr in Clinical Isolates of Enterobacteriaceae from Nine Teaching Hospitals in China. Antimicrob. Agents Chemother. 52: 4268-4273 [Abstract] [Full Text]  
  • Shimizu, K., Kikuchi, K., Sasaki, T., Takahashi, N., Ohtsuka, M., Ono, Y., Hiramatsu, K. (2008). Smqnr, a New Chromosome-Carried Quinolone Resistance Gene in Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 52: 3823-3825 [Abstract] [Full Text]  
  • Cesaro, A., Bettoni, R. R. D., Lascols, C., Merens, A., Soussy, C. J., Cambau, E. (2008). Low selection of topoisomerase mutants from strains of Escherichia coli harbouring plasmid-borne qnr genes. J Antimicrob Chemother 61: 1007-1015 [Abstract] [Full Text]  
  • Arsene, S., Leclercq, R. (2007). Role of a qnr-Like Gene in the Intrinsic Resistance of Enterococcus faecalis to Fluoroquinolones. Antimicrob. Agents Chemother. 51: 3254-3258 [Abstract] [Full Text]  
  • Cattoir, V., Poirel, L., Nordmann, P. (2007). Plasmid-Mediated Quinolone Resistance Determinant QnrB4 Identified in France in an Enterobacter cloacae Clinical Isolate Coexpressing a QnrS1 Determinant. Antimicrob. Agents Chemother. 51: 2652-2653 [Full Text]  
  • Poirel, L., Leviandier, C., Nordmann, P. (2006). Prevalence and Genetic Analysis of Plasmid-Mediated Quinolone Resistance Determinants QnrA and QnrS in Enterobacteriaceae Isolates from a French University Hospital. Antimicrob. Agents Chemother. 50: 3992-3997 [Abstract] [Full Text]  
  • Turner, A. K., Nair, S., Wain, J. (2006). The acquisition of full fluoroquinolone resistance in Salmonella Typhi by accumulation of point mutations in the topoisomerase targets. J Antimicrob Chemother 58: 733-740 [Abstract] [Full Text]  
  • Bonemann, G., Stiens, M., Puhler, A., Schluter, A. (2006). Mobilizable IncQ-Related Plasmid Carrying a New Quinolone Resistance Gene, qnrS2, Isolated from the Bacterial Community of a Wastewater Treatment Plant.. Antimicrob. Agents Chemother. 50: 3075-3080 [Abstract] [Full Text]  
  • Robicsek, A., Strahilevitz, J., Sahm, D. F., Jacoby, G. A., Hooper, D. C. (2006). qnr Prevalence in Ceftazidime-Resistant Enterobacteriaceae Isolates from the United States.. Antimicrob. Agents Chemother. 50: 2872-2874 [Abstract] [Full Text]  
  • Kehrenberg, C., Friederichs, S., de Jong, A., Michael, G. B., Schwarz, S. (2006). Identification of the plasmid-borne quinolone resistance gene qnrS in Salmonella enterica serovar Infantis. J Antimicrob Chemother 58: 18-22 [Abstract] [Full Text]  
  • Jacoby, G. A., Walsh, K. E., Mills, D. M., Walker, V. J., Oh, H., Robicsek, A., Hooper, D. C. (2006). qnrB, Another Plasmid-Mediated Gene for Quinolone Resistance.. Antimicrob. Agents Chemother. 50: 1178-1182 [Abstract] [Full Text]  
  • Poirel, L., Pitout, J. D. D., Calvo, L., Rodriguez-Martinez, J.-M., Church, D., Nordmann, P. (2006). In Vivo Selection of Fluoroquinolone-Resistant Escherichia coli Isolates Expressing Plasmid-Mediated Quinolone Resistance and Expanded-Spectrum {beta}-Lactamase.. Antimicrob. Agents Chemother. 50: 1525-1527 [Abstract] [Full Text]