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Antimicrobial Agents and Chemotherapy, July 2005, p. 3050-3052, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3050-3052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction of the Plasmid-Encoded Quinolone Resistance Protein QnrA with Escherichia coli Topoisomerase IV

John H. Tran,1 George A. Jacoby,2* and David C. Hooper1

Division of Infectious Diseases, Massachusetts General Hospital, Boston,1 Infectious Disease Department, Lahey Clinic, Burlington, Massachusetts2

Received 3 February 2005/ Returned for modification 10 March 2005/ Accepted 14 April 2005

Purified QnrA blocked ciprofloxacin inhibition of topoisomerase IV, just as QnrA was previously found to prevent quinolone inhibition of DNA gyrase. With a gel displacement assay, tagged QnrA was shown to bind to topoisomerase IV and its subunits in a reaction that did not depend on the presence of DNA, quinolone, or ATP.

* Corresponding author. Mailing address: Lahey Clinic, 41 Mall Road, Burlington, MA 01805. Phone: (781) 744-2928. Fax: (781) 744-5486. E-mail: george.a.jacoby{at}lahey.org.

Antimicrobial Agents and Chemotherapy, July 2005, p. 3050-3052, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3050-3052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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