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Antimicrobial Agents and Chemotherapy, July 2005, p. 3066-3069, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3066-3069.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Survey of Enterobacteriaceae Producing Extended-Spectrum ß-Lactamases in a Slovak Hospital: Dominance of SHV-2a and Characterization of TEM-132

Martina Zarnayová,1,2 Eliane Siebor,1 André Péchinot,1 Jean-Marie Duez,1 Helena Bujdáková,2 Roger Labia,3 and Catherine Neuwirth1*

Laboratoire de Bactériologie, Hôpital Universitaire du Bocage, BP 77908, 21079 Dijon Cedex, France,1 Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia,2 Unité FRE 2125, CNRS, UBO, MNHN, 6 rue de l'Université, 29000 Quimper, France3

Received 23 November 2004/ Returned for modification 8 February 2005/ Accepted 8 April 2005

Eighty-five extended-spectrum ß-lactamase-producing Enterobacteriaceae from a Slovak hospital have been studied. SHV-2a was predominant, but other variants have been detected, namely, SHV-5, SHV-12, TEM-12, TEM-15, and TEM-132, which differed from TEM-1 by amino acid substitutions R164H, E240K, and I173V and had kinetic properties similar to those of TEM-28.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Universitaire du Bocage, BP 77908, 21079 Dijon Cedex, France. Phone: 33-3 80 29 32 60. Fax: 33-3 80 29 36 67. E-mail: catherine.neuwirth{at}chu-dijon.fr.

Antimicrobial Agents and Chemotherapy, July 2005, p. 3066-3069, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3066-3069.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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