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Antimicrobial Agents and Chemotherapy, July 2005, p. 3073-3079, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3073-3079.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals

Carla Novais,1 João C. Sousa,2 Teresa M. Coque,3 Luísa V. Peixe,1* and The Portuguese Resistance Study Group

REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal,1 Microbiologia, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Porto, Portugal,2 Servicio de Microbiologia, Hospital Ramón y Cajal, Madrid, Spain3

Received 22 December 2004/ Returned for modification 29 January 2005/ Accepted 19 March 2005

Fifty-one pulsed-field gel electrophoresis types and 17 Tn1546 variants were identified among 101 Enterococcus faecium isolates recovered in three distant Portuguese hospitals. Intra- and interhospital dissemination of specific strains and Tn1546 types was detected, which might largely contribute to the endemicity of vancomycin-resistant E. faecium in Portuguese hospitals, as happened previously in other geographical locations.

* Corresponding author. Mailing address: Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4050-030 Porto, Portugal. Phone: 351-2-22078946. Fax: 351-2-22003977. E-mail: lpeixe{at}ff.up.pt.

Antimicrobial Agents and Chemotherapy, July 2005, p. 3073-3079, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3073-3079.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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