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Antimicrobial Agents and Chemotherapy, August 2005, p. 3575-3577, Vol. 49, No. 8
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.8.3575-3577.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Simple Histidine-Rich Protein 2 Double-Site Sandwich Enzyme-Linked Immunosorbent Assay for Use in Malaria Drug Sensitivity Testing

Harald Noedl,1,2* Jan Bronnert,3 Kritsanai Yingyuen,1 Bernhard Attlmayr,2 Herwig Kollaritsch,2 and Mark Fukuda1

Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, 316/6 Rajvithi Road, Bangkok 10400, Thailand,1 Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria,2 Angewandte Tropenmedizin und Infektionsepidemiologie, Schiffahrtmedizinisches Institut der Marine, Fachgebiet I 3, Kopperpahler Allee 120, 24119 Kronshagen, Germany3

A simple double-site sandwich enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum in vitro drug sensitivity tests based on measuring histidine-rich protein 2 (HRP2) is presented. The ELISA uses two commercial monoclonal antibodies and provides a drastically cheaper alternative to the test kits previously used in the HRP2 drug sensitivity test. The assay is simple to establish and perform. The sensitivity is comparable and the drug sensitivity results very closely match those obtained with the commercial ELISA kits (R2 = 0.979; P < 0.001; mean log difference at the 50% inhibitory concentration = 0.07).

* Corresponding author. Mailing address: Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria. Phone: 43-1-4277-64885. Fax: 43-1-403 83 43 90. E-mail: harald.noedl{at}univie.ac.at.

Antimicrobial Agents and Chemotherapy, August 2005, p. 3575-3577, Vol. 49, No. 8
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.8.3575-3577.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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