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Antimicrobial Agents and Chemotherapy, August 2005, p. 3578-3582, Vol. 49, No. 8
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.8.3578-3582.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inducement and Reversal of Tetracycline Resistance in Escherichia coli K-12 and Expression of Proton Gradient-Dependent Multidrug Efflux Pump Genes

Miguel Viveiros,1 Ana Jesus,1 Mafalda Brito,1 Clara Leandro,1 Marta Martins,1 Diane Ordway,1,{dagger} Ana Maria Molnar,2 Joseph Molnar,2 and Leonard Amaral1*

Unit of Mycobacteriology and UPMM, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, R. Junqueira 96, 1349-008 Lisbon, Portugal,1 Institute of Medical Microbiology, Albert-Szent Gyorgyi School of Medicine, Dóm tér 10/11, 6720 Szeged, Hungary2

Received 11 November 2004/ Returned for modification 9 February 2005/ Accepted 29 April 2005

Expression of eight transporter genes of Escherichia coli K-12 and its {Delta}acrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the {Delta}acrAB tetracycline-induced strain.

* Corresponding author. Mailing address: Unit of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua Junqueira 96, 1349-008 Lisbon, Portugal. Phone: 351 21 365 2653. Fax: 351 21 363 2105. E-mail: lamaral{at}ihmt.unl.pt.

{dagger} Present address: Colorado State University, Department of Microbiology, Immunology and Pathology, Mycobacteria Research Laboratory, 1682 Campus Delivery, 200 West St., Fort Collins, CO 80523-1682.

Antimicrobial Agents and Chemotherapy, August 2005, p. 3578-3582, Vol. 49, No. 8
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.8.3578-3582.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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