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Antimicrobial Agents and Chemotherapy, September 2005, p. 3743-3748, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3743-3748.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

BEL-1, a Novel Clavulanic Acid-Inhibited Extended-Spectrum ß-Lactamase, and the Class 1 Integron In120 in Pseudomonas aeruginosa

Laurent Poirel,1 Laura Brinas,1,2 Annemie Verlinde,3 Louis Ide,3 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France,1 Area de Bioquimica y Biologia Molecular, Universidad de La Rioja, 26006 Logrono, Spain,2 Department of Microbiology, Heilig Hartziekenhuis Roeselare, Wilgenstraat 2, 8800 Roeselare, Belgium3

Received 16 April 2005/ Returned for modification 31 May 2005/ Accepted 1 July 2005

Screening by a double-disk synergy test identified a Pseudomonas aeruginosa isolate that produced a clavulanic acid-inhibited expanded-spectrum ß-lactamase (ESBL). Cloning and sequencing identified a novel ESBL, BEL-1, weakly related to other Ambler class A ESBLs. ß-Lactamase BEL-1 hydrolyzed significantly most expanded-spectrum cephalosporins and aztreonam, and its activity was inhibited by clavulanic acid, tazobactam, cefoxitin, moxalactam, and imipenem. This chromosome-encoded ESBL gene was embedded in a class 1 integron containing three other gene cassettes. In addition, this integron was bracketed by Tn1404 transposon sequences at its right end and by P. aeruginosa-specific sequences at its left end.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, September 2005, p. 3743-3748, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3743-3748.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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