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Antimicrobial Agents and Chemotherapy, September 2005, p. 3922-3925, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3922-3925.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Targeting of the Virulence Factor Acetohydroxyacid Synthase by Sulfonylureas Results in Inhibition of Intramacrophagic Multiplication of Brucella suis

Rose-Anne Boigegrain, Jean-Pierre Liautard, and Stephan Köhler*

Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, Pl. E. Bataillon, 34095 Montpellier, France

Received 26 January 2005/ Returned for modification 21 March 2005/ Accepted 2 June 2005

The acetohydroxyacid synthase (AHAS) of Brucella suis can be effectively targeted by the sulfonylureas chlorimuron ethyl and metsulfuron methyl. Growth in minimal medium was inhibited, and multiplication in human macrophages was totally abolished with 100 µM of sulfonylureas. Metsulfuron methyl-resistant mutants showed reduced viability in macrophages and reduced AHAS activity.

* Corresponding author. Mailing address: INSERM U-431, Université Montpellier II, CC 100, Place E. Bataillon, F-34095 Montpellier, France. Phone: 33 4 67 14 42 38. Fax: 33 4 67 14 33 38. E-mail: kohler{at}univ-montp2.fr.

Antimicrobial Agents and Chemotherapy, September 2005, p. 3922-3925, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3922-3925.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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