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Antimicrobial Agents and Chemotherapy, September 2005, p. 3926-3929, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3926-3929.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiparametric Assay To Screen and Dissect the Mode of Action of Anti-Human Immunodeficiency Virus Envelope Drugs

Julià Blanco,* Imma Clotet-Codina, Berta Bosch, Mercedes Armand-Ugón, Bonaventura Clotet, and José A. Esté

Retrovirology Laboratory, Fundació irsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, 08916 Badalona, Catalonia, Spain

Received 17 March 2005/ Returned for modification 27 May 2005/ Accepted 16 June 2005

A flow cytometry-based assay was used to simultaneously quantify X4 and R5 human immunodeficiency virus (HIV) envelope-mediated cell-to-cell viral transfer, cell death, and cell-to-cell fusion. In this assay, different anti-HIV envelope drugs showed characteristic inhibitory profiles for each measured parameter, allowing for the rapid identification of the mode of action of active compounds.

* Corresponding author. Mailing address: Fundació irsiCaixa, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Catalonia, Spain. Phone: 34934656374. Fax: 34934653968. E-mail: jblanco{at}irsicaixa.es.

Antimicrobial Agents and Chemotherapy, September 2005, p. 3926-3929, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3926-3929.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moncunill, G., Armand-Ugon, M., Clotet-Codina, I., Pauls, E., Ballana, E., Llano, A., Romagnoli, B., Vrijbloed, J. W., Gombert, F. O., Clotet, B., De Marco, S., Este, J. A. (2008). Anti-HIV Activity and Resistance Profile of the CXC Chemokine Receptor 4 Antagonist POL3026. Mol. Pharmacol. 73: 1264-1273 [Abstract] [Full Text]  
  • Bosch, B., Blanco, J., Pauls, E., Clotet-Codina, I., Armand-Ugon, M., Grigorov, B., Muriaux, D., Clotet, B., Darlix, J.-L., Este, J. A. (2005). Inhibition of Coreceptor-Independent Cell-to-Cell Human Immunodeficiency Virus Type 1 Transmission by a CD4-Immunoglobulin G2 Fusion Protein. Antimicrob. Agents Chemother. 49: 4296-4304 [Abstract] [Full Text]  


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