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The vanB2 Gene Cluster of the Majority of Vancomycin-Resistant Enterococcus faecium Isolates from Taiwan Is Associated with the pbp5 Gene and Is Carried by Tn5382 Containing a Novel Insertion Sequence

Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, Taipei, Taiwan, Republic of China

Received 2 February 2005/ Returned for modification 17 April 2005/ Accepted 5 June 2005

Thirty-two vanB2 Enterococcus faecium isolates were found to harbor Tn5382. Twenty-four isolates had a 1,419-bp sequence inserted within the open reading frame (ORF) C of Tn5382. This 1,419-bp sequence contained a 638-bp ORF with a 72% amino acid sequence homology with the transposase gene of IS150. Thirty isolates had the pbp5 gene linked to Tn5382.

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