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Antimicrobial Agents and Chemotherapy, January 2006, p. 348-350, Vol. 50, No. 1
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.1.348-350.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Anucleate Cell Blue Assay: a Useful Tool for Identifying Novel Type II Topoisomerase Inhibitors

Yoshihiro Oyamada,1 Hideaki Ito,1* Mika Fujimoto-Nakamura,1 Akihiko Tanitame,2 Noritaka Iwai,3,{dagger} Kazuo Nagai,4 Jun-ichi Yamagishi,1 and Masaaki Wachi3

Pharmacology & Microbiology Research Laboratories,1 Chemical Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Enoki 33-94, Suita, Osaka 564-0053,2 Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501,3 Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan4

Received 25 July 2005/ Returned for modification 17 September 2005/ Accepted 30 October 2005

About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors.

* Corresponding author. Mailing address: Pharmacology & Microbiology Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Enoki 33-94, Suita, Osaka 564-0053, Japan. Phone: 81-6-63375910. Fax: 81-6-63387656. E-mail: hideaki-ito{at}ds-pharma.co.jp.

{dagger} Present address: Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.

Antimicrobial Agents and Chemotherapy, January 2006, p. 348-350, Vol. 50, No. 1
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.1.348-350.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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