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Antimicrobial Agents and Chemotherapy, October 2006, p. 3460-3463, Vol. 50, No. 10
0066-4804/06/$08.00+0     doi:10.1128/AAC.00440-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Method for Regulated Expression of Single-Copy Efflux Pump Genes in a Surrogate Pseudomonas aeruginosa Strain: Identification of the BpeEF-OprC Chloramphenicol and Trimethoprim Efflux Pump of Burkholderia pseudomallei 1026b

Ayush Kumar,1 Kim-Lee Chua,2 and Herbert P. Schweizer1*

Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682,1 Department of Biochemistry, National University of Singapore, Singapore2

Received 7 April 2006/ Returned for modification 18 June 2006/ Accepted 21 July 2006

Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.

* Corresponding author. Mailing address: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682. Phone: (970) 491-3536. Fax: (970) 491-1815. E-mail: Herbert.Schweizer{at}colostate.edu.

Antimicrobial Agents and Chemotherapy, October 2006, p. 3460-3463, Vol. 50, No. 10
0066-4804/06/$08.00+0     doi:10.1128/AAC.00440-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lopez, C. M., Rholl, D. A., Trunck, L. A., Schweizer, H. P. (2009). Versatile Dual-Technology System for Markerless Allele Replacement in Burkholderia pseudomallei. Appl. Environ. Microbiol. 75: 6496-6503 [Abstract] [Full Text]  
  • Chan, Y. Y., Bian, H. S., Tan, T. M. C., Mattmann, M. E., Geske, G. D., Igarashi, J., Hatano, T., Suga, H., Blackwell, H. E., Chua, K. L. (2007). Control of Quorum Sensing by a Burkholderia pseudomallei Multidrug Efflux Pump. J. Bacteriol. 189: 4320-4324 [Abstract] [Full Text]  


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