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Antimicrobial Agents and Chemotherapy, December 2006, p. 3992-3997, Vol. 50, No. 12
0066-4804/06/$08.00+0     doi:10.1128/AAC.00597-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Prevalence and Genetic Analysis of Plasmid-Mediated Quinolone Resistance Determinants QnrA and QnrS in Enterobacteriaceae Isolates from a French University Hospital{triangledown}

Laurent Poirel, Cécile Leviandier, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 94275 K.-Bicêtre, France

Received 16 May 2006/ Returned for modification 14 August 2006/ Accepted 6 September 2006

The spread of plasmid-mediated quinolone resistance determinants QnrA and QnrS was evaluated in a collection of 186 extended-spectrum ß-lactamase (ESBL)-positive enterobacterial isolates from 2002 to 2005 and 185 nalidixic acid-resistant strains isolated during the first 6 months of 2005 at the Bicêtre hospital, France. Out of these 186 ESBL-positive isolates, 2.2 and 1.6% carried a QnrA1 and a QnrS1 determinant, respectively. The ESBLs associated with QnrA1 were VEB-1, SHV-12, and CTX-M-1, whereas those associated with QnrS1 were TEM-52, SHV-12, and CTX-M-1. Among the 185 nalidixic acid-resistant strains isolated in 2005, 0.5 and 2.7% had a QnrA1 determinant and a QnrS1 determinant, respectively. The genetic environments of the qnrA1 gene differed but were always associated with sul1 type integrons. In contrast, qnrS1 genes were not embedded in class 1 integrons but located often (but not systematically) downstream of the insertion sequence ISEcl2 on plasmids that often carried a novel ß-lactamase gene, blaLAP-1. This is the first study identifying the QnrS resistance determinant in Europe and indicating that this determinant might also be widespread.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.aphp.fr.

{triangledown} Published ahead of print on 18 September 2006.

Antimicrobial Agents and Chemotherapy, December 2006, p. 3992-3997, Vol. 50, No. 12
0066-4804/06/$08.00+0     doi:10.1128/AAC.00597-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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