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Antimicrobial Agents and Chemotherapy, December 2006, p. 4189-4190, Vol. 50, No. 12
0066-4804/06/$08.00+0     doi:10.1128/AAC.00657-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of a Chromosome-Carried erm(B) Gene and Its Flanking Insertion Points in Lactobacillus johnsonii G41{triangledown}

Ana Belén Flórez, Mohammed Salim Ammor, Susana Delgado, and Baltasar Mayo*

Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300-Villaviciosa, Asturias, Spain

Received 30 May 2006/ Returned for modification 28 July 2006/ Accepted 12 September 2006

An erm(B) gene carried on the Lactobacillus johnsonii G41 chromosome and the upstream and downstream regions were fully sequenced. Apparently, a 1,495-bp segment of pRE25 from Enterococcus faecalis carrying the erm(B) gene became inserted, by an unknown mechanism, into the L. johnsonii chromosome.

* Corresponding author. Mailing address: Baltasar Mayo, Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300-Villaviciosa, Spain. Phone: 34 985 89 21 31. Fax: 34 985 89 22 33. E-mail: baltasar.mayo{at}ipla.csic.es.

{triangledown} Published ahead of print on 25 September 2006.

Antimicrobial Agents and Chemotherapy, December 2006, p. 4189-4190, Vol. 50, No. 12
0066-4804/06/$08.00+0     doi:10.1128/AAC.00657-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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