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Antimicrobial Agents and Chemotherapy, February 2006, p. 607-617, Vol. 50, No. 2
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.2.607-617.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Emergence of DHA-1-Producing Klebsiella spp. in the Parisian Region: Genetic Organization of the ampC and ampR Genes Originating from Morganella morganii

Charlotte Verdet,1,2 Yahia Benzerara,1 Valérie Gautier,2 Olivier Adam,3 Zahia Ould-Hocine,4 and Guillaume Arlet1,2*

Service de Bactériologie, Hôpital Tenon, Paris, France,1 UPRES EA 2392 UFR Saint-Antoine Université Paris VI, Paris, France,2 Service de Microbiologie Médicale, Institut Gustave Roussy, Villejuif, France,3 Service de Bactériologie, Hôpital Henri Mondor, Créteil, France4

Received 8 June 2005/ Returned for modification 27 July 2005/ Accepted 12 November 2005

Eleven Klebsiella pneumoniae clinical isolates and one Klebsiella oxytoca clinical isolate showing various pulsed-field gel electrophoresis types and producing an inducible DHA-1 class C ß-lactamase were isolated in the Parisian region between 1998 and 2003. The aim of this study was to compare the genetic organization of the blaDHA-1 genes in this collection of clinical isolates. In four isolates, the Morganella morganii-derived genomic region containing blaDHA-1 was inserted in an entire complex sul1-type integron, including a region common to In6-In7 (CR1), as previously described in a blaDHA-1-producing Salmonella enterica serovar Enteritidis KF92 isolate from Saudi Arabia in 1992. Different gene cassette arrays were characterized in each of these integrons. In two of them, an additional 10-kb fragment was inserted between the CR1 and the M. morganii-derived region and was similar to the sap (ABC transporter family) and psp (phage shock protein) operons originated from Salmonella enterica serovar Typhimurium. The length of the M. morganii region was variable, suggesting that several independent recombination events have occurred and that open reading frame orf513 encodes a recombinase involved in the mobilization of the resistance genes. The genetic organization of blaDHA-1 was identical in the eight other isolates. This structure is likely derived from a complex integron following the insertion of IS26, leading to the deletion of the first part of integron. The horizontal transfer of one plasmid carrying that truncated integron was shown for seven of these isolates.

* Corresponding author. Mailing address: Service de Bactériologie, Hôpital Tenon, 4, rue de la Chine, Paris 75970 cedex, France. Phone: 31 1 56 01 70 18. Fax: 33 1 56 01 61 08. E-mail: guillaume.arlet{at}tnn.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, February 2006, p. 607-617, Vol. 50, No. 2
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.2.607-617.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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