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Antimicrobial Agents and Chemotherapy, April 2006, p. 1594-1598, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1594-1598.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France

Jean-Winoc Decousser,1,2* Imen Methlouthi,2 Patrick Pina,3 Anne Collignon,4 Pierre Allouch,2 on behalf of the ColBVH Study Group

Laboratoire de Biologie, Centre Hospitalier de Dourdan, Dourdan, France,1 Service d'Hygiène Hospitalière, Centre Hospitalier de Versailles André Mignot, Versailles, France,2 Service de Médecine B, Hôpital de Plaisir Grignon, Plaisir Grignon, France,3 Laboratoire de Microbiologie, Centre Hospitalier Universitaire Jean Verdier, Bondy, France4

Received 3 August 2005/ Returned for modification 4 September 2005/ Accepted 5 January 2006

A real-time PCR assay with locked nucleic acid probes was developed to screen mutations at codons 79 and 83 of the Streptococcus pneumoniae parC gene. Only silent mutations were detected among 236 French invasive fluoroquinolone-susceptible strains. This test could be useful for some high-risk patients or in national surveys.

* Corresponding author. Mailing address: Laboratoire de Biologie, Centre Hospitalier de Dourdan, 2 Rue du Potelet, 91415 Dourdan, France. Phone: (33) 1 60 81 58 92. Fax: (33) 1 60 81 58 97. E-mail: jean-winoc.decousser{at}wanadoo.fr.

Antimicrobial Agents and Chemotherapy, April 2006, p. 1594-1598, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1594-1598.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Page, S., Vernel-Pauillac, F., O'Connor, O., Bremont, S., Charavay, F., Courvalin, P., Goarant, C., Le Hello, S. (2008). Real-Time PCR Detection of gyrA and parC Mutations in Streptococcus pneumoniae. Antimicrob. Agents Chemother. 52: 4155-4158 [Abstract] [Full Text]  


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