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Antimicrobial Agents and Chemotherapy, May 2006, p. 1745-1752, Vol. 50, No. 5
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.5.1745-1752.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Complex Genetic Structures with Repeated Elements, a sul-Type Class 1 Integron, and the blaVEB Extended-Spectrum ß-Lactamase Gene

Thierry Naas,1* Daniel Aubert,1 Thierry Lambert,2 and Patrice Nordmann1

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre,1 Centre d'Etudes Pharmaceutiques, Châtenay-Malabry, France2

Received 7 December 2005/ Returned for modification 3 February 2006/ Accepted 4 March 2006

Two clinical isolates of Pseudomonas aeruginosa, TL-1 and TL-2, were isolated from a patient transferred from Bangladesh and hospitalized for osteomyelitis in Paris, France. P. aeruginosa TL-1 expressed the extended-spectrum ß-lactamase VEB-1a and was susceptible only to imipenem and colistin, while P. aeruginosa TL-2 expressed only the naturally occurring blaAmpC gene at a basal level and exhibited a wild-type ß-lactam resistance phenotype. In TL-1, the typical 5'-end conserved sequence (5'-CS) region of class 1 integrons usually present upstream of the blaVEB-1a gene was replaced by a truncated 3'-CS and a 135-bp repeated element (Re). Downstream of the blaVEB-1a gene, an insertion sequence, ISPa31 disrupted by ISPa30, and an orf513 sequence, belonging to a common region (conserved region 1 [CR1]) immediately upstream of the aphA-6 gene, were present. Further downstream, a second truncated 3'-CS region in direct repeat belonged to In51, an integron containing two gene cassettes (aadA6 and the OrfD cassette). Thus, the overall structure corresponded to a sul-type class 1 integron termed In121. Genetic analyses revealed that both isolates were clonally related and differed by a ca. 100-kb fragment that contained In121. Both isolates contained another integron, In122, that carried three gene cassettes: aadB, dfrA1, and the OrfX cassette. This work identifies for the first time the spread of Re-associated blaVEB genes located on a sul-type integron. It also reports for the first time a CR1 element in P. aeruginosa that is associated with an aminoglycoside resistance aphA-6 gene that is expressed from a composite promoter.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-29-86. Fax: 33-1-45-21-63-40. E-mail: thierry.naas{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, May 2006, p. 1745-1752, Vol. 50, No. 5
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.5.1745-1752.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poirel, L., Mugnier, P. D., Toleman, M. A., Walsh, T. R., Rapoport, M. J., Petroni, A., Nordmann, P. (2009). ISCR2, Another Vehicle for blaVEB Gene Acquisition. Antimicrob. Agents Chemother. 53: 4940-4943 [Abstract] [Full Text]  
  • Zong, Z., Partridge, S. R., Iredell, J. R. (2009). A blaVEB-1 Variant, blaVEB-6, Associated with Repeated Elements in a Complex Genetic Structure. Antimicrob. Agents Chemother. 53: 1693-1697 [Abstract] [Full Text]  
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