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Antimicrobial Agents and Chemotherapy, June 2006, p. 2222-2224, Vol. 50, No. 6
0066-4804/06/$08.00+0     doi:10.1128/AAC.01443-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Effect of Culture Medium on the Disk Diffusion Method for Determining Antifungal Susceptibilities of Dermatophytes

Belkys Fernández-Torres,1 Alfonso Carrillo-Muñoz,2 Isabel Inza,1 and Josep Guarro1*

Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, 43201 Reus, Spain,1 Department of Microbiology, Asesoría Científica y de Investigación Aplicada, 08080 Barcelona, Spain2

Received 9 November 2005/ Returned for modification 21 December 2005/ Accepted 21 March 2006

We have evaluated a disk diffusion method to determine the activities of five drugs against 50 strains of dermatophytes and to assess the influence of the culture medium (antibiotic medium 3, high-resolution medium, and RPMI) on the inhibition zone diameters (IZD). There were no differences among the medium/drug combinations, except for itraconazole-RPMI, which showed the narrowest IZD.

* Corresponding author. Mailing address: Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201 Reus, Spain. Phone: 34-977-759359. Fax: 34-977-759322. E-mail: josep.guarro{at}urv.net.

Antimicrobial Agents and Chemotherapy, June 2006, p. 2222-2224, Vol. 50, No. 6
0066-4804/06/$08.00+0     doi:10.1128/AAC.01443-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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