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Antimicrobial Agents and Chemotherapy, July 2006, p. 2428-2432, Vol. 50, No. 7
0066-4804/06/$08.00+0     doi:10.1128/AAC.01561-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mechanism of Drug Resistance in Clonally Related Clinical Isolates of Vibrio fluvialis Isolated in Kolkata, India

Vijaya Bharathi Srinivasan,1,{dagger}* Rupinder Kaur Virk,1,{dagger} Amita Kaundal,1 Rupa Chakraborty,2 Basabjit Datta,2 T. Ramamurthy,2 Asish K. Mukhopadhyay,2 and Amit Ghosh1,{ddagger}

Institute of Microbial Technology, Chandigarh, India,1 National Institute of Cholera and Enteric Diseases, Kolkata, India2

Received 8 December 2005/ Returned for modification 7 March 2006/ Accepted 23 April 2006

The molecular mechanisms of drug resistance in 19 strains of Vibrio fluvialis isolated from 1998 to 2002 in Kolkata, India, were investigated. Class 1 integrons were detected in eight strains, and four strains were found to carry SXT integrases. In the presence of carbonyl cyanide m-chlorophenylhydrazone or reserpine, all nalidixic acid- and ciprofloxacin-resistant strains became sensitive, suggesting that drug efflux plays a major role in quinolone resistance in V. fluvialis. It was further seen that strains which had MICs of >25 µg/ml for nalidixic acid had a sense mutation (Ser to Ile) at position 83 of the quinolone resistance-determining region of gyrA. All except one of the integron- and SXT integrase-bearing strains belonged to the same ribotype.

* Corresponding author. Mailing address: Institute of Microbial Technology, Molecular Biology Division, Sector 39A, Chandigarh 160036, India. Phone: 91-1722-690751. Fax: 91-1722-690585. E-mail: vijaya{at}imtech.res.in.

{dagger} V. B. Srinivasan and R. K. Virk contributed equally to this work.

{ddagger} Present address: School of Biological Sciences, Indian Institute of Advanced Research (The Puri Foundation), Block 2, 1st Floor, Udyog Bhavan, Sector 11, Gandhinagar, Gujarat, India.

Antimicrobial Agents and Chemotherapy, July 2006, p. 2428-2432, Vol. 50, No. 7
0066-4804/06/$08.00+0     doi:10.1128/AAC.01561-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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