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Antimicrobial Agents and Chemotherapy, July 2006, p. 2433-2438, Vol. 50, No. 7
0066-4804/06/$08.00+0     doi:10.1128/AAC.00150-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Clonal Dissemination of a CTX-M-15 ß-Lactamase-Producing Escherichia coli Strain in the Paris Area, Tunis, and Bangui

M. Lavollay,1 K. Mamlouk,1,2 T. Frank,1,3 A. Akpabie,4 B. Burghoffer,1 S. Ben Redjeb,2 R. Bercion,3 V. Gautier,1 and G. Arlet1,5*

Laboratoire de Bactériologie, UPRES EA 2392, Faculté de Médecine Pierre et Marie Curie, Université Paris VI, Paris, France,1 Laboratoire de Microbiologie, Hôpital Charles Nicolle, Tunis, Tunisia,2 Institut Pasteur de Bangui, Bangui, République Centre Africaine,3 Unité d'Hygiène, Hôpital Emile Roux, Limeil-Brevannes, France,4 Service de Bactériologie-Hygiène, Hôpital Tenon, Paris, France5

Received 4 February 2006/ Returned for modification 31 March 2006/ Accepted 20 April 2006

One hundred twenty CTX-M-15-producing Escherichia coli strains isolated in 10 different hospitals from Paris (France), in the Hospital Charles Nicolle in Tunis (Tunisia), and in the Pasteur Institute in Bangui, Central African Republic (CAR), between 2000 and 2004 were studied. Eighty isolates, recovered from the three countries, were clonally related by repetitive extragenic palindromic PCR and pulsed-field gel electrophoresis. Various resistance profiles were identified among these clonal strains. After conjugation or electroporation of plasmids from E. coli strains representative of each profile and each geographic region, we observed seven resistance profiles in the recipient strains. Incompatibility typing showed that all the plasmids transferred from the clonal strains studied, except one, belonged to the incompatibility group FII. They all shared a multidrug resistance region (MDR) resembling the MDR region located in pC15-1a, a plasmid associated with an outbreak of a CTX-M-15-producing E. coli strain in Canada. They also shared the common backbone of an apparent mosaic plasmid, including several features present in pC15-1a and in pRSB107, a plasmid isolated from a sewage treatment plant. This study suggests that although the plasmid-borne blaCTX-M-15 gene could be transferred horizontally, its dissemination between France, Tunisia, and CAR was due primarily to its residence in an E. coli clone with a strong propensity for dissemination.

* Corresponding author. Mailing address: Service de Bactériologie-Hygiène, Hôpital Tenon, 4 rue de la Chine, 75970 Paris cedex 20, France. Phone: 33 1 56 01 70 18. Fax: 33 1 56 01 61 08. E-mail: guillaume.arlet{at}tnn.aphp.fr.

Antimicrobial Agents and Chemotherapy, July 2006, p. 2433-2438, Vol. 50, No. 7
0066-4804/06/$08.00+0     doi:10.1128/AAC.00150-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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