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Antimicrobial Agents and Chemotherapy, July 2006, p. 2493-2499, Vol. 50, No. 7
0066-4804/06/$08.00+0     doi:10.1128/AAC.01310-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

New Cluster of Plasmid-Located Class 1 Integrons in Vibrio cholerae O1 and a dfrA15 Cassette-Containing Integron in Vibrio parahaemolyticus Isolated in Angola

Daniela Ceccarelli,1 Anna Maria Salvia,1 Joana Sami,2 Piero Cappuccinelli,3 and Mauro Maria Colombo1*

Dipartimento Biologia Cellulare e dello Sviluppo, Universitá La Sapienza, Via dei Sardi 70, 00185 Rome, Italy,1 Dipartimento Scienze Biomediche, Facolta di Medicina Universitá di Sassari, Viale San Pietro 42B, 07100 Sassari, Italy,3 Institute of National Public Health, Mininistry of Health, Luanda, Angola2

Received 6 October 2005/ Returned for modification 5 December 2005/ Accepted 28 April 2006

The resistance profile and its correlation with mobile genetic elements were investigated in 11 Vibrio cholerae O1 and 2 Vibrio parahaemolyticus clinical isolates, as well as in 1 V. cholerae O1 and 1 V. cholerae non-O1 environmental isolate, isolated between 1991 and 1996 in different provinces of Angola. All clinical isolates of V. cholerae O1 were resistant to ampicillin, chloramphenicol, trimethoprim, sulfamethoxazole, and tetracycline. They also contained a large conjugative plasmid (p3iANG) with a set of three class 1 integrons harboring dfrA15, blaP1, and qacH-aadA8 cassettes, which code for resistance to trimethoprim, beta-lactams, quaternary ammonium compounds, and aminoglycosides, clustered in a 19-kb region. Chloramphenicol (cat1), kanamycin (aph), sulfonamide (sul2), and tetracycline (tetG) resistance genes were also carried on the plasmid within the same 19-kb region. A chromosomal integron containing the dfrA15 cassette was also revealed in V. parahaemolyticus strains. SXT integrase genes were present in six V. cholerae isolates but apparently were not associated with known SXT-associated resistance genes. This study indicates that plasmids and integrons contributed mainly to the circulation of multiple-drug resistance determinants in Vibrio strains from Angola.

* Corresponding author. Mailing address: Dip. Biologia Cellulare e dello Sviluppo, Univ. di Roma La Sapienza, Via dei Sardi 70, 00185 Roma, Italy. Phone: 390649917585. Fax: 390649917594. E-mail: mauro.colombo{at}uniroma1.it.

Antimicrobial Agents and Chemotherapy, July 2006, p. 2493-2499, Vol. 50, No. 7
0066-4804/06/$08.00+0     doi:10.1128/AAC.01310-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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