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Antimicrobial Agents and Chemotherapy, August 2006, p. 2700-2706, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

CTX-M-Type Extended-Spectrum ß-Lactamases in Italy: Molecular Epidemiology of an Emerging Countrywide Problem

Claudia Mugnaioli,1 Francesco Luzzaro,2 Filomena De Luca,1 Gioconda Brigante,2 Mariagrazia Perilli,3 Gianfranco Amicosante,3 Stefania Stefani,4 Antonio Toniolo,2 and Gian Maria Rossolini1*

Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Università di Siena, I-53100 Siena,1 Laboratorio di Microbiologia Ospedale di Circolo, Università dell'Insubria, I-21100 Varese,2 Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, I-67100 L'Aquila,3 Dipartimento di Scienze Microbiologiche e Ginecologiche, Università di Catania, I-95124 Catania, Italy4

Received 16 January 2006/ Returned for modification 9 March 2006/ Accepted 22 May 2006

A nationwide survey of extended-spectrum ß-lactamase (ESBL) production among Enterobacteriaceae, carried out in 2003, showed that CTX-M-type enzymes have achieved a sizeable prevalence among ESBL producers in Italy, mostly in Escherichia coli and, to a lesser extent, in Klebsiella pneumoniae. In this work, we report on the molecular epidemiology of the CTX-M-producing isolates from that survey and on the mechanisms of dissemination of these emerging resistance determinants. The CTX-M-producing isolates were detected in 10 of the 11 participating centers distributed across the Italian national territory, although at remarkably variable rates in different centers (1.2 to 49.5% of the ESBL producers). All CTX-M determinants were of group 1, with CTX-M-15 and CTX-M-1 being the most prevalent variants (60% and 35%, respectively) and CTX-M-32 carried by a minority (5%) of isolates. Each variant was detected both in E. coli and in K. pneumoniae. Genotyping of the CTX-M-producing isolates by random amplification of polymorphic DNA revealed a notable diversity, especially among those producing CTX-M-1, while clonal expansion was evident with some CTX-M-15-producing strains. Mating experiments revealed a higher overall transferability of blaCTX-M-1 and blaCTX-M-32 than of blaCTX-M-15. Coresistance to quinolones and aminoglycosides was overall higher with the CTX-M-15-producing isolates. The present results indicate that CTX-M-producing strains are now widespread across the Italian territory and underscore the emerging role of these ESBL determinants in the European setting. They also reveal notable differences in the dissemination mechanisms of genes encoding different CTX-M variants of the same lineage.

* Corresponding author. Mailing address: Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Policlinico Santa Maria alle Scotte, I-53100 Siena, Italy. Phone: 39-0577-233455. Fax: 39-0577-233334. E-mail: rossolini{at}unisi.it.

Antimicrobial Agents and Chemotherapy, August 2006, p. 2700-2706, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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