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Antimicrobial Agents and Chemotherapy, August 2006, p. 2728-2731, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00357-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

In Vitro Activities of Daptomycin, Vancomycin, and Penicillin against Clostridium difficile, C. perfringens, Finegoldia magna, and Propionibacterium acnes

Kerin L. Tyrrell,1* Diane M. Citron,1 Yumi A. Warren,1 Helen T. Fernandez,1 C. Vreni Merriam,1 and Ellie J. C. Goldstein1,2

R. M. Alden Research Lab, Santa Monica, California 90404,1 UCLA School of Medicine, Los Angeles, California 900952

Received 23 March 2006/ Returned for modification 21 April 2006/ Accepted 26 May 2006

Daptomycin has in vitro activity against gram-positive anaerobic bacteria, although limited numbers of species have been tested. We studied the in vitro activities of daptomycin, vancomycin, and penicillin against more than 100 strains each of Clostridium difficile, C. perfringens, Finegoldia magna, and Propionibacterium acnes. Daptomycin Etest MICs and results from time-kill studies were determined for selected strains. For 392 of 421 strains (93%), daptomycin was inhibitory at ≤1 µg/ml, including 15 of 16 strains of C. difficile with elevated linezolid MICs of 8 and 16 µg/ml, all 32 strains with moxifloxacin MICs of ≥4 µg/ml, and all 16 strains resistant to clindamycin. Daptomycin MICs were also ≤1 µg/ml for all 16 F. magna strains resistant to clindamycin and all 32 strains resistant to tetracycline. Only one strain, a C. perfringens strain, had a MIC of >2 µg/ml to daptomycin. Eighty-five and 92.5% of the Etest MICs were within 1 dilution of the agar dilution method for all drugs at 24 and 48 h, respectively. In time-kill studies, a C. difficile strain was inhibited by both daptomycin and vancomycin at 1, 2, 4, 8, and 24 h; colony counts were decreased by 2.3 to 2.9 log at 24 h. Vancomycin was not bactericidal for C. perfringens; however, daptomycin showed bactericidal activity as early as 1 h at four and eight times the MIC and at 2 and 4 h at two and four times the MIC.

* Corresponding author: Mailing address: R. M. Alden Research Laboratory, 2001 Santa Monica Blvd., Suite 685W, Santa Monica, CA 90404. Phone: (310) 453-7820. Fax: (310) 453-7670. E-mail: k.l.tyrrell{at}verizon.net.

Antimicrobial Agents and Chemotherapy, August 2006, p. 2728-2731, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00357-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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