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Antimicrobial Agents and Chemotherapy, August 2006, p. 2824-2828, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00167-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Contribution of Penicillin-Binding Protein Homologs to Antibiotic Resistance, Cell Morphology, and Virulence of Listeria monocytogenes EGDe

Caitriona M. Guinane,1,3,{dagger} Paul D. Cotter,1 R. Paul Ross,2,3 and Colin Hill1,3*

Department of Microbiology, University College Cork, Cork,1 Teagasc Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork,2 Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland3

Received 8 February 2006/ Returned for modification 31 March 2006/ Accepted 23 May 2006

Seven open reading frames, annotated as potential penicillin-binding-protein-encoding genes (lmo0441, lmo0540, lmo1438, lmo1892, lmo2039, lmo2229, and lmo2754), were targeted for insertional mutagenesis in Listeria monocytogenes EGDe. These genes were found to contribute in various degrees to ß-lactam resistance, cell morphology, or the virulence potential of this organism.


* Corresponding author. Mailing address: Department of Microbiology, University College Cork, Cork, Ireland. Phone: 353 21 4901373. Fax: 353 21 4903101. E-mail: c.hill{at}ucc.ie.

{dagger} Present address: The Centre for Infectious Diseases, Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, United Kingdom.


Antimicrobial Agents and Chemotherapy, August 2006, p. 2824-2828, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00167-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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