This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Assunção, P. Articles by Davey, H. M. Search for Related Content PubMed PubMed Citation Articles by Assunção, P. Articles by Davey, H. M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, August 2006, p. 2845-2849, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.01582-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Flow Cytometric Determination of the Effects of Antibacterial Agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp. capricolum, and Mycoplasma mycoides subsp. mycoides Large Colony Type

Patricia Assunção,^1* Nuno T. Antunes,¹ Ruben S. Rosales,¹ Carlos Poveda,¹ Jose B. Poveda,¹ and Hazel M. Davey²

Unidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Arucas, Spain,¹ Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD Wales, United Kingdom²

Received 13 December 2005/ Returned for modification 6 February 2006/ Accepted 13 May 2006

Flow cytometry together with SYBR green I and propidium iodide was used to study the effects of enrofloxacin, ciprofloxacin, gentamicin, chloramphenicol, oxytetracycline, and tylosin on four mycoplasma species. Inhibition of mycoplasma growth could be detected by as early as 3 h after the start of treatment. The strongest effect was observed with enrofloxacin- and ciprofloxacin-treated cells.

---

* Corresponding author. Mailing address: Unidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Trasmontaña s/n, 35416, Arucas, Las Palmas, Spain. Phone: 34 928 45 11 22. Fax: 34 928 45 11 42. E-mail: passuncao{at}becarios.ulpgc.es.

---

Antimicrobial Agents and Chemotherapy, August 2006, p. 2845-2849, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.01582-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Shubar, H. M., Mayer, J. P., Hopfenmuller, W., Liesenfeld, O. (2008). A new combined flow-cytometry-based assay reveals excellent activity against Toxoplasma gondii and low toxicity of new bisphosphonates in vitro and in vivo. J Antimicrob Chemother 61: 1110-1119 [Abstract] [Full Text]