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Antimicrobial Agents and Chemotherapy, August 2006, p. 2863-2865, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00449-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

New Integron-Associated Gene Cassette Encoding a Trimethoprim-Resistant DfrB-Type Dihydrofolate Reductase

Renee S. Levings,1,2 Diane Lightfoot,3 Liam D. H. Elbourne,4 Steven P. Djordjevic,1 and Ruth M. Hall4*

New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Microbiology and Immunology Section, Camden, NSW 2570,1 Department of Biological Sciences, University of Wollongong, NSW 2522,2 Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Victoria 3010,3 School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia4

Received 11 April 2006/ Returned for modification 6 May 2006/ Accepted 23 May 2006

A sixth gene cassette containing a dfrB-type gene, dfrB6, was found in a dfrB6-aadA1 cassette array in class 1 integrons. This array was isolated from several multiply antibiotic-resistant Salmonella enterica serovar Infantis strains that appear to be clonally related. The DfrB6 dihydrofolate reductase conferred resistance to trimethoprim.

* Corresponding author. Mailing address: School of Molecular and Microbial Biosciences, Biochemistry and Microbiology Building G08, The University of Sydney, NSW 2006, Australia. Phone: 61-2-9351-3465. Fax: 61-2-9351-4571. E-mail: Ruth.Hall{at}mmb.usyd.edu.au.

Antimicrobial Agents and Chemotherapy, August 2006, p. 2863-2865, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00449-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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