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Antimicrobial Agents and Chemotherapy, January 2007, p. 175-180, Vol. 51, No. 1
0066-4804/07/$08.00+0     doi:10.1128/AAC.01096-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin{triangledown}

Go Hirokawa,1,2 Hideko Kaji,1* and Akira Kaji2

Department of Biochemistry and Molecular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107,1 Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 191042

Received 30 August 2006/ Returned for modification 16 October 2006/ Accepted 22 October 2006

The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 mM Mg2+. Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the 30S subunits was expelled by paromomycin-induced subunit association (70S formation). These results indicate that the stabilization of 70S ribosomes by paromomycin may in part be responsible for its inhibitory effects on translocation and ribosome recycling.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, JAH 456A, Philadelphia, PA 19107. Phone: (215) 503-6547. Fax: (215) 923-7343. E-mail: Hideko.Kaji{at}Jefferson.edu.

{triangledown} Published ahead of print on 6 November 2006.

Antimicrobial Agents and Chemotherapy, January 2007, p. 175-180, Vol. 51, No. 1
0066-4804/07/$08.00+0     doi:10.1128/AAC.01096-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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  • Wang, G., Inaoka, T., Okamoto, S., Ochi, K. (2009). A Novel Insertion Mutation in Streptomyces coelicolor Ribosomal S12 Protein Results in Paromomycin Resistance and Antibiotic Overproduction. Antimicrob. Agents Chemother. 53: 1019-1026 [Abstract] [Full Text]  
  • Hirokawa, G., Iwakura, N., Kaji, A., Kaji, H. (2008). The role of GTP in transient splitting of 70S ribosomes by RRF (ribosome recycling factor) and EF-G (elongation factor G). Nucleic Acids Res 36: 6676-6687 [Abstract] [Full Text]  
  • Demeshkina, N., Hirokawa, G., Kaji, A., Kaji, H. (2007). Novel activity of eukaryotic translocase, eEF2: dissociation of the 80S ribosome into subunits with ATP but not with GTP. Nucleic Acids Res 35: 4597-4607 [Abstract] [Full Text]  


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