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Antimicrobial Agents and Chemotherapy, January 2007, p. 350-353, Vol. 51, No. 1
0066-4804/07/$08.00+0     doi:10.1128/AAC.00985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Simple Colorimetric Inhibition Assay of Heme Crystallization for High-Throughput Screening of Antimalarial Compounds{triangledown}

Nguyen Tien Huy,1,2 Dinh Thanh Uyen,1 Atsushi Maeda,1 Dai Thi Xuan Trang,1 Tatsuo Oida,3 Shigeharu Harada,1 and Kaeko Kamei1*

Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan,1 Venture Laboratory, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan,2 Department of Materials and Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan3

Received 7 August 2006/ Returned for modification 14 September 2006/ Accepted 24 October 2006

Current assays for screening new antimalarials need initiators of ß-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in ß-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of ß-hematin formation.

* Corresponding author. Mailing address: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Phone: 81-75-724-7553. Fax: 81-75-724-7541. E-mail: kame{at}kit.ac.jp.

{triangledown} Published ahead of print on 6 November 2006.

Antimicrobial Agents and Chemotherapy, January 2007, p. 350-353, Vol. 51, No. 1
0066-4804/07/$08.00+0     doi:10.1128/AAC.00985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huy, N. T., Mizunuma, K., Kaur, K., Nhien, N. T. T., Jain, M., Uyen, D. T., Harada, S., Jain, R., Kamei, K. (2007). 2-tert-Butyl-8-Quinolinamines Exhibit Potent Blood Schizontocidal Antimalarial Activity via Inhibition of Heme Crystallization. Antimicrob. Agents Chemother. 51: 2842-2847 [Abstract] [Full Text]  


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