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Antimicrobial Agents and Chemotherapy, January 2007, p. 394-396, Vol. 51, No. 1
0066-4804/07/$08.00+0     doi:10.1128/AAC.00637-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Effects of Inoculum Size and Incubation Time on Broth Microdilution Susceptibility Testing of Lactic Acid Bacteria{triangledown}

Maria Egervärn,1,2* Hans Lindmark,1 Stefan Roos,2 Geert Huys,3 and Sven Lindgren1

National Food Administration,1 Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden,2 Laboratory of Microbiology, Ghent University, Ghent, Belgium3

Received 24 May 2006/ Returned for modification 21 July 2006/ Accepted 16 October 2006

Inoculum size and incubation time were varied during broth microdilution testing of the susceptibilities of 35 strains of lactic acid bacteria to six antibiotics. An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 x 105 CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution.

* Corresponding author. Mailing address: Microbiology Division, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden. Phone: 46 18 17 53 15. Fax: 46 18 17 14 94. E-mail: mia.egervarn{at}slv.se.

{triangledown} Published ahead of print on 23 October 2006.

Antimicrobial Agents and Chemotherapy, January 2007, p. 394-396, Vol. 51, No. 1
0066-4804/07/$08.00+0     doi:10.1128/AAC.00637-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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