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Antimicrobial Agents and Chemotherapy, November 2007, p. 4171-4173, Vol. 51, No. 11
0066-4804/07/$08.00+0     doi:10.1128/AAC.01480-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Emergence of the Quinolone Resistance-Mediating Gene aac(6')-Ib-cr in Extended-Spectrum-ß-Lactamase-Producing Klebsiella Isolates Collected in Slovenia between 2000 and 2005{triangledown}

Jerneja Ambrozic Avgustin,1* Rok Keber,1 Katja Zerjavic,1 Toni Orazem,2 and Miklavz Grabnar1

Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111,1 Institute of Public Health of the Republic of Slovenia, Grabloviceva 44, 1000 Ljubljana, Slovenia2

Received 24 November 2006/ Returned for modification 15 February 2007/ Accepted 18 August 2007

Seventy-four nonrepetitive uropathogenic fluoroquinolone-resistant or -intermediate extended-spectrum-ß-lactamase-producing Klebsiella isolates from Slovenia were screened for the presence of plasmid-mediated quinolone resistance genes. None of the known qnr genes were detected. The aac(6')-Ib-cr allele was detected on plasmids from 25 transconjugants for which the ciprofloxacin MIC was higher than for the recipient Escherichia coli strain.

* Corresponding author. Mailing address: Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia. Phone: 386 1 4233388. Fax: 386 1 2573390. E-mail: Jerneja.Ambrozic{at}bf.uni-lj.si

{triangledown} Published ahead of print on 10 September 2007.

Antimicrobial Agents and Chemotherapy, November 2007, p. 4171-4173, Vol. 51, No. 11
0066-4804/07/$08.00+0     doi:10.1128/AAC.01480-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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