This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Donnio, P.-Y.
Right arrow Articles by Le Coustumier, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Donnio, P.-Y.
Right arrow Articles by Le Coustumier, A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 2007, p. 4342-4350, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.01414-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular and Epidemiological Evidence for Spread of Multiresistant Methicillin-Susceptible Staphylococcus aureus Strains in Hospitals{triangledown}

Pierre-Yves Donnio,1,2* Frédéric Février,3 Pablo Bifani,4 Marie Dehem,5 Christèle Kervégant,1 Nathalie Wilhelm,3 Anne-Lise Gautier-Lerestif,1,2 Nathalie Lafforgue,1,2 Michel Cormier,1,2 the MR-MSSA Study Group of the Collège de Bactériologie-Virologie-Hygiène des Hôpitaux de France {dagger} Alain Le Coustumier,3

Département de Bactériologie-Virologie et Hygiène Hospitalière, Centre Hospitalier Universitaire, 35033 Rennes, France,1 UPRES-1254 Microbiologie, Université Rennes I, 35043 Rennes, France,2 Laboratoire de Biologie, Centre Hospitalier, 46005 Cahors, France,3 Pasteur Institute, B-1180 Brussels, Belgium,4 Genoscreen, 59000 Lille, France5

Received 20 November 2006/ Returned for modification 26 January 2007/ Accepted 10 August 2007

The excision of the staphylococcal chromosomal cassette mec (SCCmec) from methicillin-resistant Staphylococcus aureus (MRSA) strains results in methicillin-susceptible S. aureus (MSSA) strains. In order to determine the proportion and diversity of multidrug-resistant MSSA (MR-MSSA) strains derived from MRSA strains, 247 mecA-negative isolates recovered in 60 French hospitals between 2002 and 2004 were characterized. The spa types of all strains were determined, and a subset of the strains (n = 30) was further genotyped by multilocus sequence typing. The IDI-MRSA assay was used to test the isolates for the presence of the SCCmec element, which was detected in 68% of all isolates analyzed. Molecular analysis of the samples suggested that 92% of the MR-MSSA isolates were derived from MRSA clones of diverse genetic backgrounds, of which the clone of sequence type 8 and SCCmec type IVA accounted for most of the samples. High variations in incidence data and differences in the molecular characteristics of the isolates from one hospital to another indicate that the emergence of MR-MSSA resulted from independent SCCmec excisions from epidemic MRSA isolates, as well as the diffusion of methicillin-susceptible strains after the loss of SCCmec. MR-MSSA could constitute a useful model for the study of the respective genetic and environmental factors involved in the dissemination of S. aureus in hospitals.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie, Centre Hospitalier Universitaire, 2 rue Henri Le Guilloux, 35033 Rennes, France. Phone: 33-2-99-28-42-76. Fax: 33-2-99-28-41-59. E-mail: pierre-yves.donnio{at}chu-rennes.fr

{triangledown} Published ahead of print on 20 August 2007.

{dagger} Members of the MR-MSSA Study Group are microbiologists from hospitals in Antibes (V. Blanc), Arles (K. Krechiem), Arras (M. Marcolin, M. N. Noulard), Aulnay-sous-Bois (H. Porcheret), Aurillac (M. Villemain), Avranches (D. Siep Heng, P. Deprez), Bagnols-sur-Cèze (G. Khatib), Bayeux (J. Carre, E. Heusse), Belley (O. Sabot), Béthune (S. Dekeyser, D. Descamps), Béziers (L. Gazagne), Bourg-en-Bresse (F. Laurent), Cannes (D. Neri-Schiavini, F. Carmagnol), Châlons-en-Champagne (I. Baudinat), Charleville-Mézières (C. Auvray, S. Delesalle), Dax (J. P. Lafargue), Douai (S. Hendricx), Dourdan (J. W. Decousser, A. Sarran), Dunkerque (A. Verhaeghe), Elbeuf (G. Grise), Falaise (G. Gallou), Foix-Pamiers (A. Clarac-Panisello), Fort-de-France (C. Olive, R. Theodose), Gonesse (D. Barraud, M. Terki), Langres (D. Simeon), Lannion (J. L. Laborie), La Roche-sur-Yon (G. Chambreuil, A. S. Poirier), Le Mans (A. Marmonnier, C. Varache), Lorient (J. F. Ygout), Mantes-La Jolie (L. Berardi-Grassias, F. Richardin), Marseille (P. Brunet, A. Michel), Maubeuge (M. Vasseur), Metz (J. Didion, Y. Rio), Meulan (E. Vallee), Monaco (S. Gabriel, P. Sorlin), Montceau-les-Mines (O. Menouni), Montreuil-sur-Mer (M. Menouar), Montluçon (S. Laluque), Mulhouse (J. M. Delarbre), Nemours (P. Guiet), Neufchateau (M. Thouvenin), Orléans (D. Poisson, L. Bret), Orsay (B. Ferre, B. Mounkassa), Perpignan (P. Gueudet, E. Lecaillon), Poissy (M. Leneveu, G. Rast), Provins (C. Durand, F. Pateyron), Quimper (F. Geffroy), Quimperlé (A. Valogne), Sablé-sur-Sarthe (S. Fosse-Ledinger, E. Jaouen), Sallanches (P. Clergeau), Saint-Brieuc (J. Vaucel), Saint-Denis de la Réunion (C. Denoix, M. C. Jaffar), Saint-Etienne Clinique Mutualiste (R. Meley), Saint-Germain-en-Laye (M. Cheron, E. Vallee), Saumur (E. Bichier), Seclin (C. Rolland), Sète (A. Barrans, B. Lamy), Thionville (M. Perrin), Troyes (C. Eloy), Vannes (P. Pouedras), Versailles (B. Pangon), and Vesoul (P. Chantelat).

Antimicrobial Agents and Chemotherapy, December 2007, p. 4342-4350, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.01414-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Grobner, S., Dion, M., Plante, M., Kempf, V. A. J. (2009). Evaluation of the BD GeneOhm StaphSR Assay for Detection of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Spiked Positive Blood Culture Bottles. J. Clin. Microbiol. 47: 1689-1694 [Abstract] [Full Text]  
  • Munson, E., Block, T., Voegeli, J. T., Hryciuk, J. E., Schell, R. F. (2009). Cost-Effective Frozen Master Mix Modification of a Commercial Methicillin-Resistant Staphylococcus aureus PCR Assay. J. Clin. Microbiol. 47: 1888-1891 [Abstract] [Full Text]  
  • Wolk, D. M., Picton, E., Johnson, D., Davis, T., Pancholi, P., Ginocchio, C. C., Finegold, S., Welch, D. F., de Boer, M., Fuller, D., Solomon, M. C., Rogers, B., Mehta, M. S., Peterson, L. R. (2009). Multicenter Evaluation of the Cepheid Xpert Methicillin-Resistant Staphylococcus aureus (MRSA) Test as a Rapid Screening Method for Detection of MRSA in Nares. J. Clin. Microbiol. 47: 758-764 [Abstract] [Full Text]  
  • Shore, A. C., Rossney, A. S., O'Connell, B., Herra, C. M., Sullivan, D. J., Humphreys, H., Coleman, D. C. (2008). Detection of Staphylococcal Cassette Chromosome mec-Associated DNA Segments in Multiresistant Methicillin-Susceptible Staphylococcus aureus (MSSA) and Identification of Staphylococcus epidermidis ccrAB4 in both Methicillin-Resistant S. aureus and MSSA. Antimicrob. Agents Chemother. 52: 4407-4419 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»