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Antimicrobial Agents and Chemotherapy, December 2007, p. 4462-4465, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.00455-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Negamycin Binds to the Wall of the Nascent Chain Exit Tunnel of the 50S Ribosomal Subunit

Susan J. Schroeder,^1,, Gregor Blaha,^2,3, and Peter B. Moore^1,2*

Department of Chemistry, Yale University, New Haven, Connecticut 06520-8107,¹ Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8024,² Howard Hughes Medical Institute, New Haven, Connecticut 06520-8114³

Received 3 April 2007/ Returned for modification 15 June 2007/ Accepted 24 July 2007

Negamycin, a small-molecule inhibitor of protein synthesis, binds the Haloarcula marismortui 50S ribosomal subunit at a single site formed by highly conserved RNA nucleotides near the cytosolic end of the nascent chain exit tunnel. The mechanism of antibiotic action and the function of this unexplored tunnel region remain intriguingly elusive.

Published ahead of print on 30 July 2007.

These two authors contributed equally to this work.

Present address: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019-3051.