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Antimicrobial Agents and Chemotherapy, December 2007, p. 4512-4514, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.00491-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Biochemical Characterization of SFC-1, a Class A Carbapenem-Hydrolyzing ß-Lactamase{triangledown}

Fátima Fonseca,1 Ana Cristina Sarmento,1 Isabel Henriques,1 Bart Samyn,2 Jozef van Beeumen,2 Pedro Domingues,3 Maria Rosário Domingues,3 Maria José Saavedra,4 and António Correia1*

Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal,1 Department of Biochemistry, Physiology and Microbiology, Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, Ghent, Belgium,2 Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal,3 Department of Veterinary Science, Center of Studies in Animal and Veterinary Science, University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal4

Received 11 April 2007/ Returned for modification 22 June 2007/ Accepted 20 July 2007

The carbapenem-hydrolyzing ß-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.

* Corresponding author. Mailing address: CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal. Phone: (351) 234 370 783. Fax: (351) 234 426 408. E-mail: antonio.correia{at}ua.pt

{triangledown} Published ahead of print on 17 September 2007.

Antimicrobial Agents and Chemotherapy, December 2007, p. 4512-4514, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.00491-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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