This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paraschos, S.
Right arrow Articles by Skaltsounis, A.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paraschos, S.
Right arrow Articles by Skaltsounis, A.-L.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2007, p. 551-559, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.00642-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Activities of Chios Mastic Gum Extracts and Constituents against Helicobacter pylori{triangledown}

Sotirios Paraschos,1 Prokopios Magiatis,1 Sofia Mitakou,1* Kalliopi Petraki,2 Antonios Kalliaropoulos,2 Petros Maragkoudakis,2 Andreas Mentis,2 Dionyssios Sgouras,2* and Alexios-Leandros Skaltsounis1

Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece,1 Laboratory of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 11521, Greece2

Received 25 May 2006/ Returned for modification 1 August 2006/ Accepted 10 November 2006

The extracts and pure major constituents of Chios mastic gum (resin of Pistacia lentiscus var. chia) were tested for their activities against Helicobacter pylori. A total mastic extract without polymer (TMEWP) was prepared after removal of the contained insoluble polymer in order to ameliorate solubility and enhance in vivo activity. Administration of TMEWP to H. pylori SS1-infected mice over the period of 3 months with an average dose of 0.75 mg/day led to an approximately 30-fold reduction in the H. pylori colonization (1.5 log CFU/g of tissue). However, no attenuation in the H. pylori-associated chronic inflammatory infiltration and the activity of chronic gastritis was observed. To further characterize potential active mastic constituents, the TMEWP was separated into an acidic and a neutral fraction. Both were extensively characterized by nuclear magnetic resonance and mass spectroscopy to elucidate the structure of the components contained within each fraction. After chromatographic separation, the acid fraction gave the major triterpenic acids, while the neutral fraction gave several triterpenic alcohols and aldehydes. Mastic extracts and isolated pure triterpenic acids were tested for in vitro activity against a panel of 11 H. pylori clinical strains. The acid fraction was found to be the most active extract (minimum bactericidal concentration [MBC], 0.139 mg/ml), and the most active pure compound was isomasticadienolic acid (MBC, 0.202 mg/ml [0.443 mM]). Our results show that administration of TMEWP may be effective in reducing H. pylori colonization and that the major triterpenic acids in the acid extract may be responsible for such an activity.

* Corresponding author. Mailing address for D. Sgouras: Department of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 115 21, Greece. Phone: 30210-6478824. Fax: 30210-6440171. E-mail: sgouras{at}pasteur.gr. Mailing address for S. Mitakou: Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece. Phone: 30210-7274290. Fax: 30210-7274594. E-mail: mitakou{at}pharm.uoa.gr.

{triangledown} Published ahead of print on 20 November 2006.

Antimicrobial Agents and Chemotherapy, February 2007, p. 551-559, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.00642-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • ZHOU, L., SATOH, K., TAKAHASHI, K., WATANABE, S., NAKAMURA, W., MAKI, J., HATANO, H., TAKEKAWA, F., SHIMADA, C., SAKAGAMI, H. (2009). Re-evaluation of Anti-inflammatory Activity of Mastic Using Activated Macrophages. In Vivo 23: 583-589 [Abstract] [Full Text]  
  • Loizou, S., Paraschos, S., Mitakou, S., Chrousos, G. P., Lekakis, I., Moutsatsou, P. (2009). Chios Mastic Gum Extract and Isolated Phytosterol Tirucallol Exhibit Anti-Inflammatory Activity in Human Aortic Endothelial Cells. Exp. Biol. Med. 234: 553-561 [Abstract] [Full Text]  
  • SAKAGAMI, H., KISHINO, K., KOBAYASHI, M., HASHIMOTO, K., IIDA, S., SHIMETANI, A., NAKAMURA, Y., TAKAHASHI, K., IKARASHI, T., FUKAMACHI, H., SATOH, K., NAKASHIMA, H., SHIMIZU, T., TAKEDA, K., WATANABE, S., NAKAMURA, W. (2009). Selective Antibacterial and Apoptosis-modulating Activities of Mastic. In Vivo 23: 215-223 [Abstract] [Full Text]  
  • DIMAS, K., HATZIANTONIOU, S., WYCHE, J. H., PANTAZIS, P. (2009). A Mastic Gum Extract Induces Suppression of Growth of Human Colorectal Tumor Xenografts in Immunodeficient Mice. In Vivo 23: 63-68 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»