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Antimicrobial Agents and Chemotherapy, February 2007, p. 591-596, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.01116-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Hygromycin B Inhibition of Protein Synthesis and Ribosome Biogenesis in Escherichia coli{triangledown}

Susan M. McGaha and W. Scott Champney*

Department of Biochemistry and Molecular Biology, J. H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614

Received 4 September 2006/ Returned for modification 5 October 2006/ Accepted 11 October 2006

The aminoglycoside antibiotic hygromycin B was examined in Escherichia coli cells for inhibitory effects on translation and ribosomal-subunit formation. Pulse-chase labeling experiments were performed, which verified lower rates of ribosomal-subunit synthesis in drug-treated cells. Hygromycin B exhibited a concentration-dependent inhibitory effect on viable-cell numbers, growth rate, protein synthesis, and 30S and 50S subunit formation. Unlike other aminoglycosides, hygromycin B was a more effective inhibitor of translation than of ribosomal-subunit formation in E. coli. Examination of total RNA from treated cells showed an increase in RNA corresponding to a precursor to the 16S rRNA, while mature 16S rRNA decreased. Northern hybridization to rRNA in cells treated with hygromycin B showed that RNase II- and RNase III-deficient strains of E. coli accumulated 16S rRNA fragments upon treatment with the drug. The results indicate that hygromycin B targets protein synthesis and 30S ribosomal-subunit assembly.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, J. H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614. Phone: (423) 439-2022. Fax: (423) 439-2030. E-mail: Champney{at}etsu.edu.

{triangledown} Published ahead of print on 16 October 2006.

Antimicrobial Agents and Chemotherapy, February 2007, p. 591-596, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.01116-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Maguire, B. A. (2009). Inhibition of Bacterial Ribosome Assembly: a Suitable Drug Target?. Microbiol. Mol. Biol. Rev. 73: 22-35 [Abstract] [Full Text]  
  • Champney, W. S., Rodgers, W. K. (2007). Retapamulin Inhibition of Translation and 50S Ribosomal Subunit Formation in Staphylococcus aureus Cells. Antimicrob. Agents Chemother. 51: 3385-3387 [Abstract] [Full Text]  


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