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Antimicrobial Agents and Chemotherapy, February 2007, p. 631-637, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.01082-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Novel Ambler Class A ß-Lactamase LAP-1 and Its Association with the Plasmid-Mediated Quinolone Resistance Determinant QnrS1{triangledown}

Laurent Poirel,1 Vincent Cattoir,1,2 Ana Soares,1 Claude-James Soussy,2 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris-Sud, K.-Bicêtre,1 Service de Bactériologie-Virologie, Hôpital Henri Mondor, Créteil, France2

Received 28 August 2006/ Returned for modification 8 October 2006/ Accepted 14 November 2006

The plasmid-mediated quinolone resistance determinant QnrS1 was identified in non-clonally related Enterobacter cloacae isolates in association with a transferable narrow-spectrum ß-lactam resistance marker. Cloning experiments allowed the identification of a novel Ambler class A ß-lactamase, named LAP-1. It shares 62 and 61% amino acid identity with the most closely related ß-lactamases, TEM-1 and SHV-1, respectively. It has a narrow-spectrum hydrolysis of ß-lactams and is strongly inhibited by clavulanic acid and sulbactam and, to a lesser extent, by tazobactam. Association of the blaLAP-1 gene with the qnrS1 gene was identified in E. cloacae isolates from France and Vietnam. These genes were plasmid located and associated with similar insertion sequences but were not associated with sul1-type class 1 integrons, as opposed to the qnrA genes.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.aphp.fr.

{triangledown} Published ahead of print on 20 November 2006.

Antimicrobial Agents and Chemotherapy, February 2007, p. 631-637, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.01082-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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