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Antimicrobial Agents and Chemotherapy, February 2007, p. 736-739, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.00557-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparative DNA Analysis of Two vanA Plasmids from Enterococcus faecium Strains Isolated from Poultry and a Poultry Farmer in Norway{triangledown}

H. Sletvold,1* P. J. Johnsen,1 G. S. Simonsen,2,3,4 B. Aasnæs,3 A. Sundsfjord,2,3,4 and K. M. Nielsen1,5

Department of Pharmacy,1 Department of Microbiology and Virology, Faculty of Medicine, University of Tromsø,2 Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø,3 Norwegian Institute of Public Health, Oslo,4 Norwegian Institute of Gene Ecology, Tromsø, Norway5

Received 5 May 2006/ Returned for modification 13 July 2006/ Accepted 8 November 2006

The DNA sequences of two plasmids carrying vanA, pVEF1 (39,626 bp) and pVEF2 (39,714 bp), were determined. Forty-three shared coding sequences were identified, and the only nucleotide difference was an 88-bp indel. A postsegregational killing system was identified. This system possibly explains the persistence of the vanA gene cluster in Norwegian poultry farms.

* Corresponding author. Mailing address: Department of Pharmacy, Faculty of Medicine, University of Tromsø, 9037 Tromsø, Norway. Phone: 47 77646150. Fax: 47 77646151. E-mail: hege.sletvold{at}farmasi.uit.no.

{triangledown} Published ahead of print on 20 November 2006.

Antimicrobial Agents and Chemotherapy, February 2007, p. 736-739, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.00557-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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