This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pitout, J. D. D.
Right arrow Articles by Laupland, K. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pitout, J. D. D.
Right arrow Articles by Laupland, K. B.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2007, p. 1281-1286, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01377-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of CTX-M-Producing Escherichia coli in the Calgary Health Region: Emergence of CTX-M-15-Producing Isolates{triangledown}

Johann D. D. Pitout,1,2,4* Deirdre L. Church,1,2,3 Daniel B. Gregson,1,2,3 Barbara L. Chow,1 Melissa McCracken,6 Michael R. Mulvey,6 and Kevin B. Laupland2,3,5

Division of Microbiology, Calgary Laboratory Services,1 Departments of Pathology & Laboratory Medicine,2 Medicine,3 Microbiology and Infectious Diseases,4 Critical Care, University of Calgary, Calgary, Alberta, Canada,5 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada6

Received 3 November 2006/ Returned for modification 9 January 2007/ Accepted 19 January 2007

A study was designed to describe the molecular epidemiology of CTX-M-producing Escherichia coli over a 6-year period (2000 to 2005) in a large well-defined Canadian region with a centralized laboratory system. Molecular characterization was done by isoelectric focusing, PCR, and automated sequencing, while genetic relatedness was determined by pulsed-field gel electrophoresis with XbaI. Of the 552 viable extended-spectrum ß-lactamase-producing E. coli isolates isolated, 354 (64%) were positive for blaCTX-M genes associated with ISEcp1; 211 produced CTX-M-14, 128 produced CTX-M-15, 5 produced CTX-M-2, 4 produced CTX-M-3, 4 produced CTX-M-24, and 2 produced CTX-M-27. CTX-M-positive isolates were significantly more resistant to the fluoroquinolones than CTX-M-negative isolates, while CTX-M-15 producers were more likely to be resistant to gentamicin and tobramycin. There was a predominance of CTX-M-14 during the first 4 years of the study period, with community outbreaks associated with cluster 14A during 2000, 2001, and 2003. A substantial increase in CTX-M-15 producers occurred during the last 18 months and was due to clusters 15A and 15AR (where AR indicates related to A) in the hospital and nursing home sectors. Our results demonstrate that the persistence and dissemination of CTX-M genes among E. coli populations in larger geographic health care regions is dynamic, with the continuous emergence of clonally related CTX-M-15. This study illustrates the importance of molecular surveillance in tracking CTX-M-producing E. coli strains in the community and investigating their influx into hospitals.

* Corresponding author. Mailing address: Calgary Laboratory Services, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8. Phone: (403) 770-3309. Fax: (403) 770-3347. E-mail: johann.pitout{at}cls.ab.ca

{triangledown} Published ahead of print on 5 February 2007.

Antimicrobial Agents and Chemotherapy, April 2007, p. 1281-1286, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01377-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hawkey, P. M., Jones, A. M. (2009). The changing epidemiology of resistance. J Antimicrob Chemother 64: i3-i10 [Abstract] [Full Text]  
  • Pitout, J. D. D., Gregson, D. B., Campbell, L., Laupland, K. B. (2009). Molecular Characteristics of Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Isolates Causing Bacteremia in the Calgary Health Region from 2000 to 2007: Emergence of Clone ST131 as a Cause of Community-Acquired Infections. Antimicrob. Agents Chemother. 53: 2846-2851 [Abstract] [Full Text]  
  • Johnson, J. R., Menard, M., Johnston, B., Kuskowski, M. A., Nichol, K., Zhanel, G. G. (2009). Epidemic Clonal Groups of Escherichia coli as a Cause of Antimicrobial-Resistant Urinary Tract Infections in Canada, 2002 to 2004. Antimicrob. Agents Chemother. 53: 2733-2739 [Abstract] [Full Text]  
  • Pitout, J. D. D., Campbell, L., Church, D. L., Gregson, D. B., Laupland, K. B. (2009). Molecular Characteristics of Travel-Related Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Isolates from the Calgary Health Region. Antimicrob. Agents Chemother. 53: 2539-2543 [Abstract] [Full Text]  
  • Liu, W., Chen, L., Li, H., Duan, H., Zhang, Y., Liang, X., Li, X., Zou, M., Xu, L., Hawkey, P. M. (2009). Novel CTX-M {beta}-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha, Southern China. J Antimicrob Chemother 63: 895-900 [Abstract] [Full Text]  
  • Pitout, J. D. D., Campbell, L., Church, D. L., Wang, P. W., Guttman, D. S., Gregson, D. B. (2009). Using a Commercial DiversiLab Semiautomated Repetitive Sequence-Based PCR Typing Technique for Identification of Escherichia coli Clone ST131 Producing CTX-M-15. J. Clin. Microbiol. 47: 1212-1215 [Abstract] [Full Text]  
  • Prakash, V., Lewis, J. S. II, Herrera, M. L., Wickes, B. L., Jorgensen, J. H. (2009). Oral and Parenteral Therapeutic Options for Outpatient Urinary Infections Caused by Enterobacteriaceae Producing CTX-M Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 53: 1278-1280 [Abstract] [Full Text]  
  • Leflon-Guibout, V., Blanco, J., Amaqdouf, K., Mora, A., Guize, L., Nicolas-Chanoine, M.-H. (2008). Absence of CTX-M Enzymes but High Prevalence of Clones, Including Clone ST131, among Fecal Escherichia coli Isolates from Healthy Subjects Living in the Area of Paris, France. J. Clin. Microbiol. 46: 3900-3905 [Abstract] [Full Text]  
  • Pitout, J. D. D., Wei, Y., Church, D. L., Gregson, D. B. (2008). Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6')-Ib-cr. J Antimicrob Chemother 61: 999-1002 [Abstract] [Full Text]  
  • Baudry, P. J., Nichol, K., DeCorby, M., Mataseje, L., Mulvey, M. R., Hoban, D. J., Zhanel, G. G. (2008). Comparison of Antimicrobial Resistance Profiles among Extended-Spectrum-{beta}-Lactamase-Producing and Acquired AmpC {beta}-Lactamase-Producing Escherichia coli Isolates from Canadian Intensive Care Units. Antimicrob. Agents Chemother. 52: 1846-1849 [Abstract] [Full Text]  
  • Zhanel, G. G., DeCorby, M., Laing, N., Weshnoweski, B., Vashisht, R., Tailor, F., Nichol, K. A., Wierzbowski, A., Baudry, P. J., Karlowsky, J. A., Lagace-Wiens, P., Walkty, A., McCracken, M., Mulvey, M. R., Johnson, J., The Canadian Antimicrobial Resistance Alliance (CA, , Hoban, D. J. (2008). Antimicrobial-Resistant Pathogens in Intensive Care Units in Canada: Results of the Canadian National Intensive Care Unit (CAN-ICU) Study, 2005-2006. Antimicrob. Agents Chemother. 52: 1430-1437 [Abstract] [Full Text]  
  • Valverde, A., Coque, T. M., Garcia-San Miguel, L., Baquero, F., Canton, R. (2008). Complex molecular epidemiology of extended-spectrum -lactamases in Klebsiella pneumoniae: a long-term perspective from a single institution in Madrid. J Antimicrob Chemother 61: 64-72 [Abstract] [Full Text]  
  • Lewis, J. S. II, Herrera, M., Wickes, B., Patterson, J. E., Jorgensen, J. H. (2007). First Report of the Emergence of CTX-M-Type Extended-Spectrum {beta}-Lactamases (ESBLs) as the Predominant ESBL Isolated in a U.S. Health Care System. Antimicrob. Agents Chemother. 51: 4015-4021 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»