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Antimicrobial Agents and Chemotherapy, April 2007, p. 1487-1490, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01374-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Macrolide and Tetracycline Resistances in Commensal Gemella sp. Isolates{triangledown}

Paula Cerdá Zolezzi,1 Pilar Goñi Cepero,1 Joaquim Ruiz,2 Leticia Millán Laplana,1 Carmen Rubio Calvo,1,3 and Rafael Gómez-Lus1*

Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009 Zaragoza,1 Centro de Salud Internacional, IDIBAPS, Hospital Clinic, Villarroel 170, 08036 Barcelona,2 Service of Microbiology, Hospital Clínico Lozano Blesa, C/San Juan Bosco 19, 50009 Zaragoza, Spain3

Received 3 November 2006/ Returned for modification 7 December 2006/ Accepted 19 January 2007

The epidemiologic relatedness of 29 erythromycin-resistant Gemella sp. strains from normal flora, characterized previously, were evaluated by pulsed-field gel electrophoresis (PFGE). Three isolates carried the tet(O) gene and the tet(M) gene. The msr(A) gene was found in two Gemella morbillorum strains in combination with the erm(B) or mef(E) gene. The sequences of the mef(A/E), erm(B), and msr(A) genes showed a high similarity to the corresponding sequences of other gram-positive cocci. All the strains harboring the mef(A/E) gene and the msr(D) gene possessed open reading frame 3 (ORF3)/ORF6. The 16 G. morbillorum isolates represented 15 distinct DNA profiles. Four clusters were identified (≥80% genetic relatedness). The 12 Gemella haemolysans strains belonged to different PFGE types. The clonal diversity found suggests that horizontal transfer may be the main route through which erythromycin resistance is acquired.

* Corresponding author. Mailing address: Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009 Zaragoza, Spain. Phone: 34-976-761692. Fax: 34-976-761693. E-mail: gomezlus{at}unizar.es

{triangledown} Published ahead of print on 5 February 2007.

Antimicrobial Agents and Chemotherapy, April 2007, p. 1487-1490, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01374-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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