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Antimicrobial Agents and Chemotherapy, June 2007, p. 1934-1938, Vol. 51, No. 6
0066-4804/07/$08.00+0     doi:10.1128/AAC.01028-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Phage Therapy of Pseudomonas aeruginosa Infection in a Mouse Burn Wound Model

Catherine S. McVay, Marisela Velásquez, and Joe A. Fralick^*

Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, Texas

Received 16 August 2006/ Returned for modification 21 November 2006/ Accepted 17 March 2007

Mice compromised by a burn wound injury and subjected to a fatal infection with Pseudomonas aeruginosa were administered a single dose of a Pseudomonas aeruginosa phage cocktail consisting of three different P. aeruginosa phages by three different routes: the intramuscular (i.m.), subcutaneous (s.c.), or intraperitoneal (i.p.) route. The results of these studies indicated that a single dose of the P. aeruginosa phage cocktail could significantly decrease the mortality of thermally injured, P. aeruginosa-infected mice (from 6% survival without treatment to 22 to 87% survival with treatment) and that the route of administration was particularly important to the efficacy of the treatment, with the i.p. route providing the most significant (87%) protection. The pharmacokinetics of phage delivery to the blood, spleen, and liver suggested that the phages administered by the i.p. route were delivered at a higher dose, were delivered earlier, and were delivered for a more sustained period of time than the phages administered by the i.m. or s.c. route, which may explain the differences in the efficacies of these three different routes of administration.

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* Corresponding author. Mailing address: Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430. Phone: (806) 743-2555. Fax: (806) 743-2334. E-mail: joe.fralick{at}ttmc.ttuhsc.edu

Published ahead of print on 26 March 2007.

Present address: Biological Sciences Department, Auburn University, Auburn AL 36849.

Present address: Kilimanjaro Christian Medical College, Tumaini University, P.O. Box 2240, Moshi, Tanzania, Africa.

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Antimicrobial Agents and Chemotherapy, June 2007, p. 1934-1938, Vol. 51, No. 6
0066-4804/07/$08.00+0     doi:10.1128/AAC.01028-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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