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Antimicrobial Agents and Chemotherapy, June 2007, p. 2179-2184, Vol. 51, No. 6
0066-4804/07/$08.00+0     doi:10.1128/AAC.01600-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of the Novel Narrow-Spectrum ß-Lactamase SCO-1 in Acinetobacter spp. from Argentina{triangledown}

Laurent Poirel,1,{dagger} Stéphane Corvec,1,2,{dagger} Melina Rapoport,3 Pauline Mugnier,1 Alejandro Petroni,3 Fernando Pasteran,3 Diego Faccone,3 Marcelo Galas,3 Henri Drugeon,2 Vincent Cattoir,1 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, K.-Bicêtre, Paris, France,1 Laboratoire de Bactériologie-Virologie, Hygiène Hospitalière, CHU, Nantes, France,2 Servicio Antimicrobianos, Depertamento Bacteriologia, Instituto Nacional de Enfermedades Infecciosas-ANLIS Carlos G. Malbran, Ciudad Autonoma de Buenos Aires, Argentina3

Received 22 December 2006/ Returned for modification 15 February 2007/ Accepted 23 March 2007

By studying the ß-lactamase content of several Acinetobacter spp. isolates from Argentina, producing the expanded-spectrum ß-lactamases (ESBL) VEB-1a or PER-2, a novel Ambler class A ß-lactamase gene was identified. It encoded the narrow-spectrum ß-lactamase SCO-1, whose activity was inhibited by clavulanic acid. SCO-1 hydrolyzes penicillins at a high level and cephalosporins and carbapenems at a very low level. ß-Lactamase SCO-1 was identified from unrelated VEB-1a-positive or PER-2-positive Acinetobacter spp. isolates recovered from three hospitals. The blaSCO-1 gene was apparently located on a plasmid of ca. 150 kb from all cases but was not associated with any ESBL-encoding gene. The G+C content of the blaSCO gene was 52%, a value that does not correspond to that of the A. baumannii genome (39%). ß-Lactamase SCO-1 shares 47% amino acid identity with CARB-5 and ca. 40% with the enzymes TEM, SHV, and CTX-M. A gene encoding a putative resolvase was identified downstream of the blaSCO-1 gene, but its precise way of acquisition remains to be determined.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.aphp.fr

{triangledown} Published ahead of print on 9 April 2007.

{dagger} L.P. and S.C. contributed equally to this work.

Antimicrobial Agents and Chemotherapy, June 2007, p. 2179-2184, Vol. 51, No. 6
0066-4804/07/$08.00+0     doi:10.1128/AAC.01600-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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