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Antimicrobial Agents and Chemotherapy, July 2007, p. 2420-2423, Vol. 51, No. 7
0066-4804/07/$08.00+0     doi:10.1128/AAC.01176-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

In Vitro Susceptibility of Sporothrix schenckii to Six Antifungal Agents Determined Using Three Different Methods{triangledown}

Eidi Alvarado-Ramírez and Josep M. Torres-Rodríguez*

Infectious Diseases and Mycology Research Unit (URMIM), Institut Municipal d'Investigació Mèdica (IMIM), Faculty of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain

Received 21 September 2006/ Returned for modification 28 November 2006/ Accepted 9 April 2007

The in vitro susceptibility of Sporothrix schenckii to antifungal drugs has been determined with three different methods. Nineteen Peruvian clinical isolates of S. schenckii were tested against amphotericin B (AB), flucytosine (FC), fluconazole (FZ), itraconazole (IZ), voriconazole (VZ), and ketoconazole (KZ). Modified NCCLS M38-A, Sensititre YeastOne (SYO), and ATB Fungus 2 (ATBF2) methods were used to determine the MICs. ATCC isolates of Candida parapsilosis, Candida krusei, and Aspergillus flavus were used for quality control. Sporothrix inocula were prepared with the mycelial form growing on potato dextrose agar at 28 ± 2°C. MICs of AB, FC, FZ, and IZ were determined with all three methods, VZ with M38-A and SYO, and KZ with only SYO. The three methods showed high MICs of FZ and FC (MIC90 of 0.5 µg/ml), being homogeneously lower than those of IZ and KZ. The M38-A method showed a variable MIC range of VZ (4.0 to 16 µg/ml); the geometric mean (GM) was 9.3 µg/ml. The MIC range of AB was wide (0.06 to 16 µg/ml), but the GM was 1.2 µg/ml, suggesting that the MIC is strain dependent. Agreement (two log2 dilutions) between commercial techniques and the modified M38-A method was very high with FZ, IZ, and FC. In AB and VZ, the agreement was lower, being related to the antifungal concentrations of each method. The highest activity against S. schenckii was found with IZ and KZ. Lack of activity was observed with FZ, VZ, and FC. When AB is indicated for sporotrichosis, the susceptibility of the strain must be analyzed. Commercial quantitative antifungal methods have a limited usefulness in S. schenckii.

* Corresponding author. Mailing address: Infectious Diseases and Mycology Research Unit (URMIM), Institut Municipal d'Investigació Mèdica (IMIM), Doctor Aiguader 80, E-08003 Barcelona, Spain. Phone: 34-93-2211009. Fax: 34-93-2213237. E-mail: jmtorres{at}imim.es

{triangledown} Published ahead of print on 16 April 2007.

Antimicrobial Agents and Chemotherapy, July 2007, p. 2420-2423, Vol. 51, No. 7
0066-4804/07/$08.00+0     doi:10.1128/AAC.01176-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pereira Silveira, C., Torres-Rodriguez, J. M., Alvarado-Ramirez, E., Murciano-Gonzalo, F., Dolande, M., Panizo, M., Reviakina, V. (2009). MICs and minimum fungicidal concentrations of amphotericin B, itraconazole, posaconazole and terbinafine in Sporothrix schenckii. J Med Microbiol 58: 1607-1610 [Abstract] [Full Text]  


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