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Antimicrobial Agents and Chemotherapy, August 2007, p. 2765-2773, Vol. 51, No. 8
0066-4804/07/$08.00+0     doi:10.1128/AAC.01513-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Experimental Phage Therapy against Staphylococcus aureus in Mice{triangledown} ,{dagger}

Rosanna Capparelli,1 Marianna Parlato,2 Giorgia Borriello,2 Paola Salvatore,1 and Domenico Iannelli2*

School of Biotechnological Sciences, University of Naples Federico II, Naples, Italy,1 School of Agriculture, University of Naples Federico II, Naples, Italy2

Received 1 December 2006/ Returned for modification 26 March 2007/ Accepted 8 May 2007

The present study describes a bacteriophage (MSa) active against Staphylococcus aureus, including methicillin-resistant staphylococcal strains. When inoculated into mice simultaneously with S. aureus A170 (108 CFU/mouse), phage (109 PFU) rescued 97% of the mice; when applied to nonlethal (5 x 106 CFU/mouse) 10-day infections, the phage also fully cleared the bacteria. The phage MSa, delivered inside macrophages by S. aureus, kills the intracellular staphylococci in vivo and in vitro. The phage can also prevent abscess formation and reduce the bacterial load and weight of abscesses. These results suggest a potential use of the phage for the control of both local and systemic human S. aureus infections.

* Corresponding author. Mailing address: Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Via Università 133, 80055 Portici, Naples, Italy. Phone: 39 081 2539277. Fax: 39 081 7762886. E-mail: iannelli{at}unina.it

{triangledown} Published ahead of print on 21 May 2007.

{dagger} Contribution no. DISSPAPA 146.

Antimicrobial Agents and Chemotherapy, August 2007, p. 2765-2773, Vol. 51, No. 8
0066-4804/07/$08.00+0     doi:10.1128/AAC.01513-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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